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Data S1. Materials and methods.

Figure S1. Percentage of emerged diapausing eggs in the spontaneous hatching experiment.

Figure S2. Diagnostic plot of the linear mixed effects model for “Day” during the time of adaptation for the rotifer populations.

Figure S3. Smoothers for the fitness of females over the time of adaptation extracted from the generalized additive mixed effects model.

Table S1. Results of the generalized linear mixed effects model on percentage of hatched diapausing eggs.

Table S2. The change of fitness for females over time was explored for linear or polynomial trends in the linear mixed effects model with “ordered factor Day”.

Table S3. The change of fitness for females over time was explored using “factor Day” for fitness of females.

Table S4. The generalized additive mixed effect model testing for a significant slope in fitness of females.

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