Data S1. Additional analysis of interest and not included in the manuscript.

Table S1. Museum sample information and museum accession codes. Sequence labels and mtDNA haplotype designations refer to the current study (= 54).

Table S2. Variable sites among unique haplotypes for Ciconia ciconia across = 373 bp of control region.

Table S3. Count of the regional distribution of unique haplotypes and their Genbank Accession Numbers. Haplotype codes in bold were found in the Historical sample set.

Table S4. SAShA jackknife results for all alleles including the most common (CC03) seen here as Allele 1.

Figure S1. a) Observed (OM) versus expected mean (EM) distribution of alleles when considering all haplotypes. b) Haplotype-by-haplotype analysis of the complete C. ciconia data set. The number of occurrences of each haplotype (n) and significance level (P) are given along with the haplotype specific SAShA statistics. The expected distribution is represented with circles and the observed by triangles. Allele 8 (CC040) and Allele 9 (CC005) show significant aggregation. Geographic distance (km) and Cumulative frequency of alleles describe the x and y axis, respectively.

Figure S2. Observed versus expected mean distribution of alleles with the most common haplotype (CC003) removed showing that the global distribution of CC003 overshadows some degree of underlying population structure.


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