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Details on model checking for the alternative scenarios of colonization and gene flow tested in the two steps ABC analysis (Table S1), prior distribution of temporal, demographic and mutation model parameters used in the two steps of ABC analysis (Table S1), parameter posterior distributions inferred by ABC under the A and Ahistorical scenarios, selected in first and second step, respectively (Table S3), a midpoint-rooted Maximum Likelihood Phylogeny of 83 T. thymallus mtDNA CR haplotypes based on the ML algorithm (Fig. S1) and intracluster genetic substructure analysis results (Fig. S2) are provided, together with supplementary references, as Supporting information. Microsatellite dataset, with individual information about sampling location and GenBank reference for mtDNA control region, mtDNA sequence alignment used to generate the phylogenetic tree presented in Fig. S1 and microsatellite dataset analyzed in ABC are available from the Dryad Digital Repository: http://doi.org/10.5061/dryad.p2f7c.

Table S1. Model checking for the alternative scenarios of colonization and gene flow tested in the two steps ABC analysis.

Table S2. Prior distribution of temporal, demographic, and mutation model parameters used in the two steps of ABC analysis.

Table S3. Parameter posterior distributions inferred by ABC under the A and Ahistorical scenarios, respectively, selected in first and second step.

Figure S1. Midpoint-rooted Maximum Likelihood Phylogeny of 83 T. thymallus mtDNA CR haplotypes based on the ML algorithm.

Figure S2. Intracluster genetic substructure analysis results.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.