Immunoglobulin expression in diploid and polyploid interspecies hybrids of Xenopus: evidence for allelic exclusion
Version of Record online: 16 NOV 2005
Copyright © 1983 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 13, Issue 7, pages 585–590, 1983
How to Cite
Pasquier, L. D. and Hsu, E. (1983), Immunoglobulin expression in diploid and polyploid interspecies hybrids of Xenopus: evidence for allelic exclusion. Eur. J. Immunol., 13: 585–590. doi: 10.1002/eji.1830130714
- Issue online: 16 NOV 2005
- Version of Record online: 16 NOV 2005
- Manuscript Revised: 24 MAR 1983
- Manuscript Received: 21 DEC 1982
- F. Hoffmann-La Roche and Co., Limited Company, Basel. Grant Number: CH-4005
The expression of species-specific surface IgM was studied in various Xenopus species hybrids. Whether the hybrids are diploid or polyploid, a lymphocyte does not produce all of the immunoglobulins (Ig) encloded by the Ig genes present in its genome. This suggests that the selection pressure to make a lymphocyte synthesize only one antibody is high and that allelic exclusion already existed in the ancestors of amphibians which appeared 300 million years ago.
The apparent lack of cells producing multiple Ig in polyploid hybrids having more than one pair of functional Ig genes suggests that if a stochastic model for allelic exclusion is correct, the frequency of multiple successful rearrangements has to be very low, resulting in a huge waste of lymphocyte precursors. Given the particularity of frog development it is argued that this is not likely to be the case.