Mapping cell surface antigens on mouse pre-B cell lines


  • John P. McKearn,

    Corresponding author
    1. Basel Institute for Immunology, Basel
    • Basel Institute for Immunology, Grenzacherstr. 487, CH-4005 Basel, Switzerland
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  • Naomi Rosenberg

    1. Cancer Research Center and Department of Pathology, Tufts University Medical School, Boston
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    • Supported by grants from the National Cancer Institute and the American Cancer Society and a Research Career Development Award from the National Cancer Institute.

  • The Basel Institute for Immunology was founded and is supported by F. Hoffman-La Roche Ltd., Basel, Switzerland.


Characterization of cell surface molecules expressed by B cell precursors has been hampered by the inability to routinely assay such cells from freshly explanted normal lymphoid tissues. However, a large number of transformed pre-B cell clones have been isolated which presumably include those precursors recently entering the B cell lineage (obtained from fetal liver and more rarely from adult bone marrow) and extending to those clones which are poised to become surface Ig-positive B cells. In this report, we have quantitated expression of 10 different cell surface molecules, 5 of which bear special interest as B cell differentiation antigens, among a large panel of pre-B cell clones.