Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker

Authors

  • Elisabeth Houssaint,

    Corresponding author
    1. Centre de Recherche de Biologie et de Physico-Chimie Cellulaires, Nantes
    • U 211 INSERM, Centre de Recherche de Biologie et Physico-Chimie Cellulaires, Faculté des Sciences, 2, Rue de la Houssinière, F-44072 Nantes-Cedex, France
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  • Elisabeth Diez,

    1. Centre de Recherche de Biologie et de Physico-Chimie Cellulaires, Nantes
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  • Francine V. Jotereau

    1. Centre de Recherche de Biologie et de Physico-Chimie Cellulaires, Nantes
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Abstract

A monoclonal antibody, designated T10A6, was produced by immunizing mice with H. Bl4 chicken thymocytes. T10A6, when tested by immunofluorescence, labeled 80% of thymocytes, and a subset of peripheral T cells: 20% of spleen leukocytes, 10% of blood leukocytes, 8% of bone marrow cells but less than 1% of bursal lymphocytes. Tissue distribution on polyethylene glycol sections showed that in the thymus T10A6 stained most cortical thymocytes and a portion of medullary cells. In the spleen, the positive cells appeared scattered mainly in the T-dependent areas. Ontogenic studies revealed that the antigen recognized was found in the chick embryo thymus from day 11 onward and the expression of this antigen on thymocytes reached the adult level from day 13. The first positive cells were detected in the spleen on day 13 of embryonic life. T10A6 (IgG1) precipitated a 65-kDa material from thymocytes. This is the first description of a monoclonal antibody recognizing a peripheral T cell subpopula- tion in chicken.

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