Recognition by major histocompatibility complex class I-restricted cytolytic T lymphocytes of cells expressing vaccinia-encoded viral and class I proteins

Authors

  • Marion E. Andrew,

    Corresponding author
    1. Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra
    • Australian Animal Health Laboratory, Private Bag 24, Geelong, Victoria 3220, Australia
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    • On secondment from CSIRO Australian Animal Health Laboratory, Geelong, Australia.

  • Barbara E. H. Coupar,

    1. Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra
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    • On secondment from CSIRO Australian Animal Health Laboratory, Geelong, Australia.

  • David B. Boyle,

    1. Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra
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    • On secondment from CSIRO Australian Animal Health Laboratory, Geelong, Australia.

  • Robert V. Blanden

    1. Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra
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Abstract

Target cells expressing influenza virus hemagglutinin (HA) could be recognized by cytotoxic T lymphocytes (CTL) in conjunction with the murine major histocompatibility complex class I antigen, H-2Kd, when both antigens were encoded by recombinant vaccinia virus. This recognition occurred if HA and H-2Kd were encoded by separate vaccinia viruses following dual infection of target cells or if HA and H-2Kd were encoded by a single recombinant virus. In contrast, target cells expressing nucleoprotein (NP) were only recognized by H-2Kd-restricted CTL if both NP and H-2Kd were encoded by the same vaccinia virus. These results show that the requirements for association of H-2Kd with different viral antigens derived from HA or NP can vary. Possible factors contributing to this difference are discussed.

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