Recipient of a Fulbright-Hays Fellowship.
Immunogenicity of multiple antigen peptides (MAP) containing T and B cell epitopes of the repeat region of the P. falciparum circumsporozoite protein
Article first published online: 6 DEC 2005
Copyright © 1991 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 21, Issue 12, pages 3015–3020, December 1991
How to Cite
Munesinghe, D. Y., Clavijo, P., Calle, M. C., Nussenzweig, R. S. and Nardin, E. (1991), Immunogenicity of multiple antigen peptides (MAP) containing T and B cell epitopes of the repeat region of the P. falciparum circumsporozoite protein. Eur. J. Immunol., 21: 3015–3020. doi: 10.1002/eji.1830211217
- Issue published online: 6 DEC 2005
- Article first published online: 6 DEC 2005
- Manuscript Revised: 26 AUG 1991
- Manuscript Received: 23 JUN 1991
- National Institutes of Health. Grant Number: AI 25085
- Agency for International Development. Grant Number: DPE-5979-A-00-0006-00
The immunogenicity of multiple antigen peptides (MAP) constructs containing T and B cell epitopes of the repeat region of the P. falciparum circumsporozoite (CS) protein was examined in vitro, using a human T cell clone, and in vivo, using four different strains of mice. All the MAP constructs that contained the T cell epitope, (DPNANPNVDPNANPNV), stimulated proliferation and interferon-γ production by a human T cell clone specific for this epitope which is located in the 5′ end of the repeat region of the P. falciparum CS protein. These human T cells did not recognize MAP that contained only the B cell epitope, (NANP)3, which is located in the 3′ repeat region. Optimal antibody responses were obtained in mice immunized with MAP containing four copies of tandemly arranged T and B cell epitopes, (TB)4. The murine immune response to the MAP constructs was genetically restricted. Mice of a high responder strain, C57BL, recognized both the 5′ and 3′ repeat sequences in the MAP as T, as well as B, cell epitopes and developed very high anti-MAP and anti-sporozoite antibody titers. A/J and C3H mice, which were intermediate responders, developed lower antibody titers which varied according to the orientation of the T vs. the B cell epitopes within the MAP constructs. BALB/c mice were nonresponders and did not develop antibodies following immunization with any of the MAP constructs containing the 5′ and 3′ repeats of the P. falciparum CS protein.