Human IgE mediates stimulus secretion coupling in rat basophilic leukemia cells transfected with the α chain of the human high-affinity receptor

Authors

  • A. Penelope M. Wilson,

    Corresponding author
    1. Euro/DPC Ltd., Glyn Rhonwy, Llanberis, Caernarfon, The University, Sheffield
    • Euro/DPC Ltd. Glyn Rhonwy, Llanberis, Caernarfon, Gwynedd, LL55 4EL, GB
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  • Christine E. Pullar,

    1. Department of Molecular Biology and Biotechnology, Krebs Institute for Biomolecular Research, The University, Sheffield
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    • Recipient of a SERC CASE award.

  • Andrew M. Camp,

    1. Euro/DPC Ltd., Glyn Rhonwy, Llanberis, Caernarfon, The University, Sheffield
    Current affiliation:
    1. British Biotechnology. Abingdon, OX14 3YS, GB
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  • Birgit A. Helm

    1. Euro/DPC Ltd., Glyn Rhonwy, Llanberis, Caernarfon, The University, Sheffield
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Abstract

Cell surface expression of the a chain of the human (h) Fee receptor type 1 (FceR1) can be observed following stable transfection of RBL-2H3 cells with the ha chain of the FceR1 complex. Association and dissociation constants for hIgE are similar to those previously reported for the ligand and its receptor. The demonstration of mediator release following the cross-linking of hFcϵR1α by hIgE and antigen or anti-hFcϵR1α antibody suggests that this system will assist the identification of structural motifs and specific amino acids that are involved in the generation of the signal(s) that will initiate and/or propagate the secretion of mediators from mast cells and basophils.

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