Recipient of a SERC CASE award.
Human IgE mediates stimulus secretion coupling in rat basophilic leukemia cells transfected with the α chain of the human high-affinity receptor
Article first published online: 14 DEC 2005
Copyright © 1993 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 23, Issue 1, pages 240–244, January 1993
How to Cite
Wilson, A. P. M., Pullar, C. E., Camp, A. M. and Helm, B. A. (1993), Human IgE mediates stimulus secretion coupling in rat basophilic leukemia cells transfected with the α chain of the human high-affinity receptor. Eur. J. Immunol., 23: 240–244. doi: 10.1002/eji.1830230137
- Issue published online: 14 DEC 2005
- Article first published online: 14 DEC 2005
- Manuscript Revised: 29 SEP 1992
- Manuscript Received: 8 AUG 1992
- MRC Studentship
- Human IgE;
- Rat basophilic leukemia cell line
Cell surface expression of the a chain of the human (h) Fee receptor type 1 (FceR1) can be observed following stable transfection of RBL-2H3 cells with the ha chain of the FceR1 complex. Association and dissociation constants for hIgE are similar to those previously reported for the ligand and its receptor. The demonstration of mediator release following the cross-linking of hFcϵR1α by hIgE and antigen or anti-hFcϵR1α antibody suggests that this system will assist the identification of structural motifs and specific amino acids that are involved in the generation of the signal(s) that will initiate and/or propagate the secretion of mediators from mast cells and basophils.