Thymic epithelium induces full tolerance to skin and heart but not to B lymphocyte grafts
Version of Record online: 17 NOV 2005
Copyright © 1995 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 25, Issue 2, pages 438–445, February 1995
How to Cite
Thomas-Vaslin, V., Salaün, J., Gajdos, B., le Douarin, N., Coutinho, A. and Bandeira, A. (1995), Thymic epithelium induces full tolerance to skin and heart but not to B lymphocyte grafts. Eur. J. Immunol., 25: 438–445. doi: 10.1002/eji.1830250220
- Issue online: 17 NOV 2005
- Version of Record online: 17 NOV 2005
- Manuscript Accepted: 30 NOV 1994
- Manuscript Revised: 14 NOV 1994
- Manuscript Received: 2 FEB 1994
- Thymic epithelium;
- B lymphocyte grafts
Athymic nude mice reconstituted at birth with allogeneic thymic epithelia (TE) from day 10 embryos (E10), show life-long specific tolerance to skin and heart grafts, but eliminate B lymphocytes of the TE donor haplotype, nearly as well as those from a third strain. Previous immunizations with B cells do not alter the state of tolerance to skin grafts, but specifically accelerate elimination of lymphocytes. In contrast, transplantation of E15 allogeneic thymuses already seeded by hematopoietic cells resulted in chimeras tolerant to both skin and B lymphocytes. In vitro reactivities towards stimulator spleen cells of the haplotype of the thymus were observed in both E10 TE and E15 thymus chimeras.
We conclude that induction of full in vivo tolerance to B cells requires hematopoietic cells, while this is not the case for induction of tolerance to skin and heart tissues; furthermore, in vitro reactivity to stimulator spleen cells of the tolerized haplotype is independent of in vivo tolerance.