Previously known as Bianca M. Conti-Tronconi.
Epitopes for human CD4+ cells on diphtheria toxin: Structural features of sequence segments forming epitopes recognized by most subjects
Article first published online: 23 NOV 2005
Copyright © 1995 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 25, Issue 12, pages 3207–3214, December 1995
How to Cite
Raju, R., Navaneetham, D., Okita, D., Diethelm-Okita, B., McCormick, D. and Conti-Fine, B. M. (1995), Epitopes for human CD4+ cells on diphtheria toxin: Structural features of sequence segments forming epitopes recognized by most subjects. Eur. J. Immunol., 25: 3207–3214. doi: 10.1002/eji.1830251202
- Issue published online: 23 NOV 2005
- Article first published online: 23 NOV 2005
- Manuscript Accepted: 6 SEP 1995
- Manuscript Revised: 7 AUG 1995
- Manuscript Received: 2 MAY 1995
- NINCDS. Grant Number: NS 23919 to B.M.C.-F.
- Universal T helper epitopes;
- Diphtheria toxin;
The sequence regions of diphtheria toxin (DTX) recognized by CD4+ T cells of seven healthy humans of different major histocompatibility complex haplotypes were identified. Overlapping synthetic peptides, screening the DTX sequence, were used to test in proliferation assays unselected blood CD4+ cells, or DTX-specific CD4+ lines propagated by stimulation with DTX of blood mononuclear cells. Blood CD4+ cells and DTX-specific CD4+ lines gave consistent results. Although each subject had an individual pattern of peptide recognition, six peptide sequences (residues 271–290, 321–340, 331–350, 351–370, 411–430 and 431–450) were recognized by all subjects. In the native DTX molecule, these sequence regions are flanked by sequence loops exposed on the DTX surface. They overlap uncharged segments of the DTX sequence. These structural properties may be general requirements for immunodominance in CD4+ cell sensitization in humans.