Article

A study of complexes of class II invariant chain peptide: Major histocompatibility complex class II molecules using a new complex-specific monoclonal antibody

  1. Susan Eastman1,2,*,
  2. Michael Deftos2,
  3. Paul C. Deroos1,2,
  4. Di-Hwei Hsu3,
  5. Luc Teyton4,
  6. Ned S. Braunstein5,
  7. Charles J. Hackett3,
  8. Alexander Rudensky1,2,*

Article first published online: 23 NOV 2005

DOI: 10.1002/eji.1830260218

Issue

European Journal of Immunology

European Journal of Immunology

Volume 26, Issue 2, pages 385–393, February 1996

Additional Information

How to Cite

Eastman, S., Deftos, M., Deroos, P. C., Hsu, D.-H., Teyton, L., Braunstein, N. S., Hackett, C. J. and Rudensky, A. (1996), A study of complexes of class II invariant chain peptide: Major histocompatibility complex class II molecules using a new complex-specific monoclonal antibody. Eur. J. Immunol., 26: 385–393. doi: 10.1002/eji.1830260218

Author Information

  1. 1

    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, USA

  2. 2

    Department of Immunology, University of Washington School of Medicine, Seattle, USA

  3. 3

    Immulogic Pharmaceutical Corporation, Palo Alto, USA

  4. 4

    Department of Immunology, Research Institute of Scripps Clinic, La Jolla, USA

  5. 5

    Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, USA

*Howard Hughes Medical Institute Research Laboratories, University of Washington, SLIS, Seattle, WA 98195, USA (Fax: +1-206-543-0858)

Publication History

  1. Issue published online: 23 NOV 2005
  2. Article first published online: 23 NOV 2005
  3. Manuscript Accepted: 20 NOV 1995
  4. Manuscript Revised: 10 NOV 1995
  5. Manuscript Received: 18 MAY 1995

Funded by

  • Howard Hughes Medical Institute, an NIH

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Keywords:

  • Major histocompatibility complex class II;
  • Invariant chain

Abstract

Complexes of major histocompatibility complex (MHC) class II molecules containing invariant chain (Ii)-derived peptides, known as class II-associated invariant chain peptides (CLIP), are expressed at high levels in presentation-deficient mutant cells. Expression of these complexes in mutant and wild-type antigen-presenting cells suggests that they represent an essential intermediate in the MHC class II antigen-presenting pathway. We have generated a monoclonal antibody, 30-2, which is specific for these complexes. Using this antibody, we have found quantitative differences in CLIP: MHC class II surface expression in mutant and wild-type cells. Our experiments also show that CLIP: MHC class II complexes are preferentially expressed on the cell surface similar to total mature MHC class II molecules. These complexes are found to accumulate in the endosomal compartment in the process of endosomal Ii degradation. Analysis of the fine specificity of the antibody indicates that these complexes have Ii peptide bound to the peptide-binding groove.

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