Interleukin-10 prevents the generation of dendritic cells from human peripheral blood mononuclear cells cultured with interleukin-4 and granulocyte/ macrophage-colony-stimulating factor

Authors

  • Christel Buelens,

    1. Department of Immunology, Hǒpital Erasme and Laboratory of Experimental Immunology, Faculty of Medicine, Université Libre de Bruxelles, Belgium
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  • Valérie Verhasselt,

    1. Department of Immunology, Hǒpital Erasme and Laboratory of Experimental Immunology, Faculty of Medicine, Université Libre de Bruxelles, Belgium
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  • Donat De Groote,

    1. Biosource Europe, Fleurus, Belgium
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  • Kris Thielemans,

    1. Laboratory of Physiology-Immunology, Vrije Universiteit Brussel, Belgium
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  • Michel Goldman,

    Corresponding author
    1. Department of Immunology, Hǒpital Erasme and Laboratory of Experimental Immunology, Faculty of Medicine, Université Libre de Bruxelles, Belgium
    • Department of Immunology, Hǒpital Erasme, 808, route de Lennik, B-1070 Brussels, Belgium Fax: +32.2-5 55-44 99
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  • Fabienne Willems

    1. Department of Immunology, Hǒpital Erasme and Laboratory of Experimental Immunology, Faculty of Medicine, Université Libre de Bruxelles, Belgium
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Abstract

We evaluated the effects of interleukin (IL)-10 on the differentiation of dendritic cells (DC) obtained by culturing plastic-adherent peripheral blood mononuclear cells for 7 days in presence of granulocyte/macrophage-colony-stimulating factor (GM-CSF)+IL-4. The addition of IL-10 at the initiation of culture resulted in the generation of macrophage-like cells with expressing high levels of CD14 and decreased levels of CD1a and CD1c. Furthermore, cells generated in presence of IL-10 secreted lower levels of IL-12, but higher levels of IL-8 compared with DC generated in absence of IL-10, both spontaneously and after CD40 engagement. Finally, cells generated in presence of IL-10 were less efficient than DC in stimulating the production of IL-2, interferon-γ, and IL-4 by allogeneic T cells. We conclude that IL-10 prevents the generation of DC induced by GM-CSF+IL-4 and favors the development of macrophages with a lower T cell stimulatory potential, but secreting higher levels of IL-8 than DC.

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