Heligmosomoides polygyrus inhibits established colitis in IL-10-deficient mice

Authors

  • David E. Elliott,

    Corresponding author
    1. Department of Internal Medicine, Division of Gastroenterology-Hepatology, University of Iowa College of Medicine, Iowa City, USA
    • Division of Gastroenterology (4611 JCP), University of Iowa College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242-1009, USA, Fax: +1-319-353-6399
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  • Tommy Setiawan,

    1. Department of Internal Medicine, Division of Gastroenterology-Hepatology, University of Iowa College of Medicine, Iowa City, USA
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  • Ahmed Metwali,

    1. Department of Internal Medicine, Division of Gastroenterology-Hepatology, University of Iowa College of Medicine, Iowa City, USA
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  • Arthur Blum,

    1. Department of Internal Medicine, Division of Gastroenterology-Hepatology, University of Iowa College of Medicine, Iowa City, USA
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  • Joseph F. Urban Jr.,

    1. Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA
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  • Joel V. Weinstock

    1. Department of Internal Medicine, Division of Gastroenterology-Hepatology, University of Iowa College of Medicine, Iowa City, USA
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Abstract

Inflammatory bowel disease (IBD) is prevalent in industrialized countries, but rare in less-developed countries. Helminths, common in less-developed countries, may induce immunoregulatory circuits protective against IBD. IL-10–/– mice given piroxicam develop severe and persistent colitis. Lamina propria mononuclear cells from colitic IL-10–/– mice released IFN-γ and IL-12. The ongoing piroxicam-induced colitis could be partially blocked with anti-IL-12 monoclonal antibody suggesting that the inflammation was at least partly IL-12 dependent. Colonization of piroxicam-treated colitic IL-10–/– mice with Heligmosomoides polygyrus (an intestinal helminth) suppressed established inflammation and inhibited mucosal IL-12 and IFN-γ production. H. polygyrus augmented mucosal IL-13, but not IL-4 or IL-5 production. Transfer of mesenteric lymph node (MLN) T cells from IL-10–/– animals harboring H. polygyrus into colitic IL-10–/– recipients inhibited colitis. MLN T cells from worm-free mice did not. Foxp3 (scurfin) drives regulatory T cell function. H. polygyrus enhanced Foxp3 mRNA expression in MLN T cells that had regulatory activity. This suggests that H. polygyrus inhibits ongoing IL-10–/– colitis in part through blocking mucosal Th1 cytokine production. Resolution of inflammation is associated with increased IL-13 production and can be adoptively transferred by MLN T cells.

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