The first two authors contributed equally to this work.
Humoral immune response to citrullinated collagen type II determinants in early rheumatoid arthritis
Article first published online: 15 APR 2005
Copyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 35, Issue 5, pages 1643–1652, May 2005
How to Cite
Burkhardt, H., Sehnert, B., Bockermann, R., Engström, Å., Kalden, Jochen R. and Holmdahl, R. (2005), Humoral immune response to citrullinated collagen type II determinants in early rheumatoid arthritis. Eur. J. Immunol., 35: 1643–1652. doi: 10.1002/eji.200526000
- Issue published online: 22 APR 2005
- Article first published online: 15 APR 2005
- Manuscript Accepted: 15 MAR 2005
- Manuscript Revised: 7 MAR 2005
- Manuscript Received: 12 JAN 2005
- Rheumatoid arthritis
Collagen type II (CII) is a relevant joint-specific autoantigen in the pathogenesis of rheumatoid arthritis (RA). Whereas the reasons for the breakage of self tolerance to this major cartilage component are still enigmatic, T cell responses to glycosylated CII determinants in RA patients indicate that post-translational modifications play a role. Since the conversion of arginine into citrulline by peptidylarginine deiminases (PAD) in some non-joint-specific antigens such as filaggrin or fibrin has been shown to give rise to RA-specific humoral immune responses, we investigated whether PAD modification of cartilage-specific CII might affect its recognition by circulating autoantibodies in early RA. In vitro treatment with purified PAD led to arginine deimination of native CII or of synthetic CII peptides as evidenced by amino acid analysis. The citrullination resulted in modified recognition of the immunodominant CII epitope C1III (amino acid residues 359–369) by murine and human antibodies. In a cohort of early RA patients (n=286), IgG antibodies directed toward a synthetic citrullinated C1III peptide (citC1III-P) were detectable with a prevalence of 40.4%. The partial autoantibody cross-reactivity between citC1III-P and citrullinated peptides mimicking epitopes of the cytoskeletal autoantigen filaggrin suggests that autoimmunity to cartilage-specific modified self might be a critical intermediate bridging recognition of PAD-modified extra-articular autoantigens with the disruption of tolerance to native cartilage constituents.