• 1
    Nussenzweig, V. and Nussenzweig, R. S., Rationale for the development of an engineered sporozoite malaria vaccine. Adv. Immunol. 1989. 45: 283334.
  • 2
    Tsuji, M. and Zavala, F., T cells as mediators of protective immunity against liver stages of Plasmodium. Trends Parasitol. 2003. 19: 8893.
  • 3
    Vanderberg, J. P., Nussenzweig, R. S., Most, H. and Orton, C. G., Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. II. Effects of radiation on sporozoites. J. Parasitol. 1968. 54: 11751180.
  • 4
    Suhrbier, A., Winger, L. A., Castellano, E. and Sinden, R. E., Survival and antigenic profile of irradiated malarial sporozoites in infected liver cells. Infect. Immun. 1990. 58: 28342839.
  • 5
    Belnoue, E., Costa, F. T., Frankenberg, T., Vigario, A. M., Voza, T., Leroy, N., Rodrigues, M. M. et al., Protective T cell immunity against malaria liver stage after vaccination with live sporozoites under chloroquine treatment. J. Immunol. 2004. 172: 24872495.
  • 6
    Ocaña-Morgner, C., Mota, M. and Rodriguez, A., Malaria blood-stage suppression of liver-stage immunity by dendritic cells. J. Exp. Med. 2003. 197: 143151.
  • 7
    Orjih, A. U., Cochrane, A. H. and Nussenzweig, R. S., Comparative studies on the immunogenicity of infective and attenuated sporozoites of Plasmodium berghei. Trans. R. Soc. Trop. Med. Hyg. 1982. 76: 5761.
  • 8
    Alger, N. E. and Harant, J., Plasmodium berghei: Heat-treated sporozoite vaccination of mice. Exp. Parasitol. 1976. 40: 261268.
  • 9
    Spitalny, G. L. and Nussenzweig, R., Effects of various routes of immunization and methods of parasite attenuation on the development of protection against sporozoite-induced rodent malaria. Proc. Helminthol. Soc. 1972. 39: 506514.
  • 10
    Bruna-Romero, O., Hafalla, J. C., Gonzalez-Aseguinolaza, G., Sano, G., Tsuji, M. and Zavala, F., Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR. Int. J. Parasitol. 2001. 31: 14991502.
  • 11
    Sano, G., Hafalla, J. C., Morrot, A., Abe, R., Lafaille, J. J. and Zavala, F., Swift development of protective effector functions in naive CD8(+) T cells against malaria liver stages. J. Exp. Med. 2001. 194: 173180.
  • 12
    Hafalla, J. C., Sano, G., Carvalho, L. H., Morrot, A. and Zavala, F., Short-term antigen presentation and single clonal burst limit the magnitude of the CD8(+) T cell responses to malaria liver stages. Proc. Natl. Acad. Sci. USA 2002. 99: 1181911824.
  • 13
    Kaech, S. M., Hemby, S., Kersh, E. and Ahmed, R., Molecular and functional profiling of memory CD8 T cell differentiation. Cell 2002. 111: 837851.
  • 14
    Morrot, A., Hafalla, J. C., Cockburn, I. A., Carvalho, L. H. and Zavala, F., IL-4 receptor expression on CD8+ T cells is required for the development of protective memory responses against liver stages of malaria parasites. J. Exp. Med. 2005. 202: 551560.
  • 15
    Masopust, D., Vezys, V., Usherwood, E. J., Cauley, L. S., Olson, S., Marzo, A. L., Ward, R. L. et al., Activated primary and memory CD8 T cells migrate to nonlymphoid tissues regardless of site of activation or tissue of origin. J. Immunol. 2004. 172: 48754882.
  • 16
    Murata, K., Garcia-Sastre, A., Tsuji, M., Rodrigues, M., Rodriguez, D., Rodriguez, J. R., Nussenzweig, R. S. et al., Characterization of in vivo primary and secondary CD8+ T cell responses induced by recombinant influenza and vaccinia viruses. Cell. Immunol. 1996. 173: 96107.
  • 17
    Bruna-Romero, O., Gonzalez-Aseguinolaza, G., Hafalla, J. C., Tsuji, M. and Nussenzweig, R. S., Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen. Proc. Natl. Acad. Sci. USA 2001. 98: 1149111496.
  • 18
    Oliveira-Ferreira, J., Miyahira, Y., Layton, G. T., Savage, N., Esteban, M., Rodriguez, D., Rodriguez, J. R. et al., Immunogenicity of Ty-VLP bearing a CD8(+) T cell epitope of the CS protein of P. yoelii: Enhanced memory response by boosting with recombinant vaccinia virus. Vaccine 2000. 18: 18631869.
  • 19
    Krzych, U., Schwenk, R., Guebre-Xabier, M., Sun, P., Palmer, D., White, K. and Chalom, I., The role of intrahepatic lymphocytes in mediating protective immunity induced by attenuated Plasmodium berghei sporozoites. Immunol. Rev. 2000. 174: 123134.
  • 20
    Leiriao, P., Mota, M. M. and Rodriguez, A., Apoptotic Plasmodium-infected hepatocytes provide antigens to liver dendritic cells. J. Infect. Dis. 2005. 191: 15761581.
  • 21
    Dunachie, S. J. and Hill, A. V., Prime-boost strategies for malaria vaccine development. J. Exp. Biol. 2003. 206: 37713779.
  • 22
    Luke, T. C. and Hoffman, S. L., Rationale and plans for developing a non-replicating, metabolically active, radiation-attenuated Plasmodium falciparum sporozoite vaccine. J. Exp. Biol. 2003. 206: 38033808.
  • 23
    Mueller, A. K., Labaied, M., Kappe, S. H. and Matuschewski, K., Genetically modified Plasmodium parasites as a protective experimental malaria vaccine. Nature 2005. 433: 164167.
  • 24
    Hafalla, J. C., Morrot, A., Sano, G., Milon, G., Lafaille, J. J. and Zavala, F., Early self-regulatory mechanisms control the magnitude of CD8+ T cell responses against liver stages of murine malaria. J. Immunol. 2003. 171: 964970.
  • 25
    Rodrigues, M., Li, S., Murata, K., Rodriguez, D., Rodriguez, J. R., Bacik, I., Bennink, J. R. et al., Influenza and vaccinia viruses expressing malaria CD8+ T and B cell epitopes. Comparison of their immunogenicity and capacity to induce protective immunity. J. Immunol. 1994. 153: 46364648.
  • 26
    Miyahira, Y., Murata, K., Rodriguez, D., Rodriguez, J. R., Esteban, M., Rodrigues, M. M. and Zavala, F., Quantification of antigen specific CD8+ T cells using an ELISPOT assay. J. Immunol. Methods 1995. 181: 4554.
  • 27
    Carvalho, L. H., Hafalla, J. C. and Zavala, F., ELISPOT assay to measure antigen-specific murine CD8(+) T cell responses. J. Immunol. Methods 2001. 252: 207218.
  • 28
    Romero, P., Maryanski, J. L., Cordey, A. S., Corradin, G., Nussenzweig, R. S. and Zavala, F., Isolation and characterization of protective cytolytic T cells in a rodent malaria model system. Immunol. Lett. 1990. 25: 2731.
  • 29
    Goossens, P. L., Jouin, H., Marchal, G. and Milon, G., Isolation and flow cytometric analysis of the free lymphomyeloid cells present in murine liver. J. Immunol. Methods 1990. 132: 137144.