Immunomodulation

Resident enteric microbiota and CD8+ T cells shape the abundance of marginal zone B cells

  1. Bo Wei1,
  2. Thomas T. Su2,
  3. Harnisha Dalwadi1,
  4. Robert P. Stephan3,
  5. Daisuke Fujiwara1,
  6. Tiffany T. Huang1,
  7. Sarah Brewer1,
  8. Lindy Chen1,
  9. Moshe Arditi4,
  10. James Borneman5,
  11. David J. Rawlings6,*,
  12. Jonathan Braun1,*

Article first published online: 13 NOV 2008

DOI: 10.1002/eji.200838432

Issue

European Journal of Immunology

European Journal of Immunology

Volume 38, Issue 12, pages 3411–3425, December 2008

Additional Information

How to Cite

Wei, B., Su, T. T., Dalwadi, H., Stephan, R. P., Fujiwara, D., Huang, T. T., Brewer, S., Chen, L., Arditi, M., Borneman, J., Rawlings, D. J. and Braun, J. (2008), Resident enteric microbiota and CD8+ T cells shape the abundance of marginal zone B cells. Eur. J. Immunol., 38: 3411–3425. doi: 10.1002/eji.200838432

Author Information

  1. 1

    Department of Pathology and Laboratory Medicine, UCLA David Geffen School of Medicine, Los Angeles, CA, USA

  2. 2

    Molecular Biology Institute, UCLA David Geffen School of Medicine, Los Angeles, CA, USA

  3. 3

    Division of Developmental and Clinical Immunology, University of Alabama, Birmingham, AL, USA

  4. 4

    Department of Medicine, Division of Pediatric Infectious Diseases and Immunology, Cedars-Sinai Medical Center, Los Angeles, CA, USA

  5. 5

    Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA

  6. 6

    Department of Pediatrics and Immunology, University of Washington, Seattle, WA, USA

*Seattle Children's Hospital Research Institute, 1900 9th Avenue, Seattle, WA 98101, USA, Fax: +1206-987-7310

Publication History

  1. Issue published online: 27 NOV 2008
  2. Article first published online: 13 NOV 2008
  3. Manuscript Accepted: 26 SEP 2008
  4. Manuscript Revised: 14 AUG 2008
  5. Manuscript Received: 21 APR 2008

Funded by

  • NIH. Grant Number: P30 DK349870
  • NIH. Grant Numbers: DK46763, DK69434, AI52031, HD37091, CA016042
  • Crohn's and Colitis Foundation of America
  • Broad Medical Research Foundation
  • Elizabeth Campbell Endowment

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Keywords:

  • Animal models;
  • B-cell development;
  • CD8 T cells;
  • Enteric microbiota

Abstract

Since enteric microbial composition is a distinctive and stable individual trait, microbial heterogeneity may confer lifelong, non-genetic differences between individuals. Here we report that C57BL/6 mice bearing restricted flora microbiota, a distinct but diverse resident enteric microbial community, are numerically and functionally deficient in marginal zone (MZ) B cells. Surprisingly, MZ B-cell levels are minimally affected by germ-free conditions or null mutations of various TLR signaling molecules. In contrast, MZ B-cell depletion is exquisitely dependent on cytolytic CD8+ T cells, and includes targeting of a cross-reactive microbial/endogenous MHC class 1B antigen. Thus, members of certain enteric microbial communities link with CD8+ T cells as a previously unappreciated mechanism that shapes innate immunity dependent on innate-like B cells.

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