Immunomodulation

IL-4-mediated fine tuning of IL-12p70 production by human DC

  1. Emmanuella Guenova1,
  2. Thomas Volz1,
  3. Karin Sauer1,
  4. Susanne Kaesler1,
  5. Martin R. Müller2,
  6. Florian Wölbing1,
  7. KoMing Chen1,
  8. Christoph Schwärzler3,
  9. Peter Brossart4,
  10. Martin Röcken1,
  11. Tilo Biedermann1,*

Article first published online: 16 OCT 2008

DOI: 10.1002/eji.200838463

Issue

European Journal of Immunology

European Journal of Immunology

Volume 38, Issue 11, pages 3138–3149, November 2008

Additional Information

How to Cite

Guenova, E., Volz, T., Sauer, K., Kaesler, S., Müller, M. R., Wölbing, F., Chen, K., Schwärzler, C., Brossart, P., Röcken, M. and Biedermann, T. (2008), IL-4-mediated fine tuning of IL-12p70 production by human DC. Eur. J. Immunol., 38: 3138–3149. doi: 10.1002/eji.200838463

Author Information

  1. 1

    Department of Dermatology, Eberhard Karls University, Tübingen, Germany

  2. 2

    Center for Blood Research, Harvard Medical School, Boston, MA, USA

  3. 3

    Novartis Institutes for Biomedical Research, Vienna, Austria

  4. 4

    University of Bonn, Department of Haematology and Oncology, Bonn, Germany

*Department of Dermatology, Eberhard Karls University, Liebermeisterstr. 25, 72076 Tübingen, Germany Fax: +49-7071-29-4117

Publication History

  1. Issue published online: 7 NOV 2008
  2. Article first published online: 16 OCT 2008
  3. Manuscript Accepted: 27 AUG 2008
  4. Manuscript Revised: 17 AUG 2008
  5. Manuscript Received: 27 APR 2008

Funded by

  • Deutsche Forschungsgemeinschaft. Grant Number: DFG Bi 696/3-1; SFB 685
  • Landesstiftung Baden-Württemberg. Grant Number: P-LS-AL/17
  • Fortüne Program of the University of Tübingen. Grant Numbers: F126810, 1261150)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (711K)

Keywords:

  • Cytokines;
  • DC;
  • Human;
  • Inflammation;
  • Skin

Abstract

IL-4 is expressed at high levels in allergic diseases and dominates the early phases of multiple acquired immune responses. However, the precise role of IL-4 during early inflammation and its impact on the differentiation of newly recruited DC precursors remains elusive. In order to characterize the impact of IL-4 on the differentiation of human DC, we investigated the role of IL-4 on the differentiation of monocytes into DC. Human DC were differentiated from peripheral blood precursors under either low or high concentrations of IL–4. We analyzed their cytokine profile and capacity to polarize T-cell differentiation. Concentrations of 5 (low) and 50 (high) ng/mL IL-4 induced two distinct types of DC. DC differentiated under low-dose IL-4 (5 ng/mL) produced almost no IL–12p70, and primed naïve CD4+ T cells allowing IL-4 secretion and Th2 induction. In contrast, DC generated under high concentrations of IL-4 (50 ng/mL) produced large amounts of IL-12p70, low IL-10 and primed naïve CD4+ T cells to become Th1 cells. Thus, we demonstrate that the Th2 cell cytokine IL-4 decisively determines the phenotype of ongoing immune responses by orchestrating the functional phenotype of newly immigrating DC precursors.

View Full Article (HTML) Get PDF (711K)