Immunity to infection

Passive immunization with monoclonal antibody against a 70-kDa putative adhesin of Sporothrix schenckii induces protection in murine sporotrichosis

  1. Rosana C. Nascimento1,
  2. Noeli M. Espíndola1,
  3. Rafaela A. Castro2,
  4. Pedro A. C. Teixeira2,
  5. Carla V. Loureiro y Penha2,
  6. Leila M. Lopes-Bezerra2,
  7. Sandro R. Almeida1,*

Article first published online: 7 NOV 2008

DOI: 10.1002/eji.200838513

Issue

European Journal of Immunology

European Journal of Immunology

Volume 38, Issue 11, pages 3080–3089, November 2008

Additional Information

How to Cite

Nascimento, R. C., Espíndola, N. M., Castro, R. A., Teixeira, P. A. C., Loureiro y Penha, C. V., Lopes-Bezerra, L. M. and Almeida, S. R. (2008), Passive immunization with monoclonal antibody against a 70-kDa putative adhesin of Sporothrix schenckii induces protection in murine sporotrichosis. Eur. J. Immunol., 38: 3080–3089. doi: 10.1002/eji.200838513

Author Information

  1. 1

    Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, CEP, Brazil

  2. 2

    Laboratório de Micologia Celular e Proteômica, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, São Paulo, CEP, Brazil

*Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Avenida Prof. Lineu Prestes, 580, Bloco 17, São Paulo, Brazil, CEP: 05508-900 Fax: +55-11-3813-2197

Publication History

  1. Issue published online: 7 NOV 2008
  2. Article first published online: 7 NOV 2008
  3. Manuscript Accepted: 26 AUG 2008
  4. Manuscript Revised: 25 JUL 2008
  5. Manuscript Received: 15 MAY 2008

Funded by

  • Fundação de Amparo a Pesquisa do Estado de São Paulo. Grant Number: (FAPESP Process: 06/50472-2)
  • CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnológico

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Keywords:

  • Adhesins;
  • Mab;
  • Passive immunization;
  • Sporothrix schenckii;
  • Sporotrichosis

Abstract

Cell-mediated and innate immunity are considered the most important mechanisms of host defense against fungus infections. However, recent studies demonstrated that specific antibodies show different degrees of protection against mycosis. In a previous study, antigens secreted by Sporothrix schenckii induced a specific humoral response in infected animals, mainly against the 70-kDa molecule, indicating a possible participation of antibodies to this antigen in infection control. In the present study, an IgG1 mAb was produced against a 70-kDa glycoprotein of S. schenckii in order to better understand the effect of passive immunization of mice infected with S. schenckii. Results showed a significant reduction in the number of CFU in organs of mice when the mAb was injected before and during S. schenckii infection. Similar results were observed when T-cell-deficient mice were used. Moreover, in a second schedule treatment, the mAb was injected after infection was established, and again we observed a significant reduction in CFU associated with an increase of IFN-γproduction. Also, the 70-kDa antigen is shown to be a putative adhesin present on the surface of this fungus. In conclusion, we report for the first time the protective effect of a specific antibody against S. schenckii.

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