Immunity to infection

CD4+CD25+ Treg induction by an HSP60-derived peptide SJMHE1 from Schistosoma japonicum is TLR2 dependent

  1. Xuefeng Wang1,
  2. Sha Zhou1,
  3. Ying Chi1,
  4. Xiaoyun Wen1,
  5. Jason Hoellwarth1,
  6. Lei He1,
  7. Feng Liu1,
  8. Calvin Wu2,
  9. Shawn Dhesi2,
  10. Jiaqing Zhao1,
  11. Wei Hu3,
  12. Chuan Su1,*

Article first published online: 30 OCT 2009

DOI: 10.1002/eji.200939335

Issue

European Journal of Immunology

European Journal of Immunology

Volume 39, Issue 11, pages 3052–3065, November 2009

Additional Information

How to Cite

Wang, X., Zhou, S., Chi, Y., Wen, X., Hoellwarth, J., He, L., Liu, F., Wu, C., Dhesi, S., Zhao, J., Hu, W. and Su, C. (2009), CD4+CD25+ Treg induction by an HSP60-derived peptide SJMHE1 from Schistosoma japonicum is TLR2 dependent. Eur. J. Immunol., 39: 3052–3065. doi: 10.1002/eji.200939335

Author Information

  1. 1

    Department of Pathogen Biology & Immunology, Department of Pharmacology, Jiangsu Key Laboratory of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, P. R. China

  2. 2

    Department of Educational Affairs, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

  3. 3

    National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, P. R. China

*Department of Pathogen Biology & Immunology, Department of Pharmacology, Jiangsu Key Laboratory of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, P. R. China Fax: +86-25-86862774

Publication History

  1. Issue published online: 30 OCT 2009
  2. Article first published online: 30 OCT 2009
  3. Manuscript Accepted: 24 AUG 2009
  4. Manuscript Revised: 5 AUG 2009
  5. Manuscript Received: 14 FEB 2009

Funded by

  • National Basic Research Program of P. R. China (973 Program). Grant Number: 2007CB513106
  • National Natural Science Foundation of P. R. China. Grant Numbers: 30571629, 30872206, BK2007533, 07KJA31023
  • Jiangsu Province

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Keywords:

  • CD4+CD25+ Treg;
  • Immunomodulation;
  • Schistosomes;
  • SJMHE1;
  • TLR2

Abstract

Chronic schistosome infection results in the suppression of host immune responses, allowing long-term schistosome survival and restricting pathology. Current theories suggest that Treg play an important role in this regulation. However, the mechanism of Treg induction during schistosome infection is still unknown. The aim of this study was to determine the mechanism behind the induction of CD4+CD25+ T cells by Schistosoma japonicum HSP60 (SjHSP60)-derived peptide SJMHE1 as well as to elucidate the cellular and molecular basis for the induction of CD4+CD25+ T cells during S. japonicum infection. Mice immunized with SJMHE1 or spleen and LN cells from naïve mice pretreated with SJMHE1 in vitro all displayed an increase in CD4+CD25+ T-cell populations. Release of IL-10 and TGF-β by SJMHE1 stimulation may contribute to suppression. Adoptively transferred SJMHE1-induced CD4+CD25+ T cells inhibited delayed-type hypersensitivity in BALB/c mice. Additionally, SJMHE1-treated APC were tolerogenic and induced CD4+ cells to differentiate into suppressive CD4+CD25+ Treg. Furthermore, our data support a role for TLR2 in SJMHE1-mediated CD4+CD25+ Treg induction. These findings provide the basis for a more complete understanding of the S. japonicum–host interactions that contribute to host homeostatic mechanisms, preventing an excessive immune response.

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