Contrasting roles of macrophages and dendritic cells in controlling initial pulmonary Brucella infection

Authors

  • Cristel Archambaud,

    Corresponding author
    1. Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France
    2. INSERM, U631, Marseille, France
    3. CNRS, UMR6102, Marseille, France
    • Centre d'Immunologie de Marseille-Luminy, Case 906, 13288 Marseille Cedex 09, France Fax: +33-4-91-26-94-30
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  • Suzana P. Salcedo,

    1. Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France
    2. INSERM, U631, Marseille, France
    3. CNRS, UMR6102, Marseille, France
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  • Hugues Lelouard,

    1. Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France
    2. INSERM, U631, Marseille, France
    3. CNRS, UMR6102, Marseille, France
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  • Elisabeth Devilard,

    1. Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France
    2. INSERM, U631, Marseille, France
    3. CNRS, UMR6102, Marseille, France
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  • Beatrice de Bovis,

    1. Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France
    2. INSERM, U631, Marseille, France
    3. CNRS, UMR6102, Marseille, France
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  • Nico Van Rooijen,

    1. Department of Molecular Cell Biology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands
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  • Jean-Pierre Gorvel,

    1. Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France
    2. INSERM, U631, Marseille, France
    3. CNRS, UMR6102, Marseille, France
    Current affiliation:
    1. Unité des Interactions Bactéries-Cellules, INSERM, U604, INRA USC2020, Institut Pasteur, France
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  • Bernard Malissen

    Corresponding author
    1. Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France
    2. INSERM, U631, Marseille, France
    3. CNRS, UMR6102, Marseille, France
    • Centre d'Immunologie de Marseille-Luminy, Case 906, 13288 Marseille Cedex 09, France Fax: +33-4-91-26-94-30
    Search for more papers by this author

Abstract

Control of pulmonary pathogens constitutes a challenging task as successful immune responses need to be mounted without damaging the lung parenchyma. Using immunofluorescence microscopy and flow cytometry, we analyzed in the mouse the initial innate immune response that follows intranasal inoculation of Brucella abortus. Bacteria were absent from parenchymal dendritic cells (DC) but present in alveolar macrophages in which they replicated. When the number of alveolar macrophages was reduced prior to Brucella infection, small numbers of pulmonary DC were infected and a massive recruitment of TNF-α- and iNOS-producing DC ensued. Coincidentally, Brucella disseminated to the lung-draining mediastinal lymph nodes (LN) where they replicated in both migratory DC and migratory alveolar macrophages. Together, these results demonstrate that alveolar macrophages are critical regulators of the initial innate immune response against Brucella within the lungs and show that pulmonary DC and alveolar macrophages play rather distinct roles in the control of microbial burden.

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