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Figure S1. Gating Doublets were excluded by FSC-H versus FSC-A and lymphocytes identified by size and granularity (FSC-A versus SSC-A). Viable lymphocytes were selected on the basis of exclusion of live/dead aqua stain. Total T cells were identified as CD3+ viable lymphocytes and iNKT cells as either 6B11+CD3+ or tetramer+CD3+ cells. Fluorescence minus one controls with isotype control antibodies were used to establish gating for iNKT cells and CD161, CD4 and CD8.

Figure S2. Gating strategy for identification of B cells and monocytes. Doublets were excluded by FSC-H versus FSC-A and viable cells were selected on the basis of exclusion of live/dead aqua. B cells were identified as CD19+SSClow and monocytes as CD14+SSCmid. CD1d expression was determined by MFI of the PE channel and specificity determined by fluorescence minus one with isotype control antibody controls.

Figure S3. Example of human CD1d expression on EBV-B cells after transfection. EBV-B cells were identified on the basis of size (FSC vs SSC) and dead cell excluded with the use of a viability dye, human CD1d was detected at the cell surface with a fluorescent antibody.

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