The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures

Authors

  • Fréderic Pont,

    1. INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France
    2. Université Toulouse III Paul-Sabatier, Toulouse, France
    3. ERL 5294 CNRS, BP3028, Hospital Purpan, Toulouse, France
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    • These authors contributed equally to this work.

  • Julien Familiades,

    1. INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France
    2. Université Toulouse III Paul-Sabatier, Toulouse, France
    3. ERL 5294 CNRS, BP3028, Hospital Purpan, Toulouse, France
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    • These authors contributed equally to this work.

  • Sébastien Déjean,

    1. Institut de Mathématiques, Université Toulouse III Paul-Sabatier, Toulouse, France
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  • Séverine Fruchon,

    1. INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France
    2. Université Toulouse III Paul-Sabatier, Toulouse, France
    3. ERL 5294 CNRS, BP3028, Hospital Purpan, Toulouse, France
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  • Delphine Cendron,

    1. INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France
    2. Université Toulouse III Paul-Sabatier, Toulouse, France
    3. ERL 5294 CNRS, BP3028, Hospital Purpan, Toulouse, France
    Current affiliation:
    1. Department of Microbiology & Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
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  • Mary Poupot,

    1. INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France
    2. Université Toulouse III Paul-Sabatier, Toulouse, France
    3. ERL 5294 CNRS, BP3028, Hospital Purpan, Toulouse, France
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  • Rémy Poupot,

    1. INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France
    2. Université Toulouse III Paul-Sabatier, Toulouse, France
    3. ERL 5294 CNRS, BP3028, Hospital Purpan, Toulouse, France
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  • Fatima L'Faqihi-Olive,

    1. Université Toulouse III Paul-Sabatier, Toulouse, France
    2. INSERM UMR1043, Center of Physiopathology of Toulouse Purpan, Toulouse, France
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  • Nais Prade,

    1. Department of Haematology, Hospital Purpan, Toulouse, France
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  • Bernard Ycart,

    1. Laboratoire Jean Kuntzmann, Université de Grenoble Joseph Fourier and CNRS, Grenoble, France
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  • Jean-Jacques Fournié

    Corresponding author
    1. INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France
    2. Université Toulouse III Paul-Sabatier, Toulouse, France
    3. ERL 5294 CNRS, BP3028, Hospital Purpan, Toulouse, France
    • INSERM UMR1037, Cancer Research Center of Toulouse, 31024 Toulouse, France Fax: +33-56-2745858

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Abstract

Global transcriptional technologies have revolutionised the study of lymphoid cell populations, but human γδ T lymphocytes specific for phosphoantigens remain far less deeply characterised by these methods despite the great therapeutic potential of these cells. Here we analyse the transcriptome of circulating TCRVγ+ γδ T cells isolated from healthy individuals, and their relation with those from other lymphoid cell subsets. We report that the gene signature of phosphoantigen-specific TCRVγ+ γδ T cells is a hybrid of those from αβ T and NK cells, with more ‘NK-cell’ genes than αβ T cells have and more ‘T-cell’ genes than NK cells. The expression profile of TCRVγ+ γδ T cells stimulated with phosphoantigen recapitulates their immediate physiological functions: Th1 cytokine, chemokine and cytotoxic activities reflect their high mitotic activity at later time points and do not indicate antigen-presenting functions. Finally, such hallmarks make the transcriptome of γδ T cells, whether resting or clonally expanding, clearly distinctive from that of NK/T or peripheral T-cell lymphomas of the γδ subtype.

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