Intraendosomal flow cytometry: A novel approach to analyze the protein composition of antigen-loaded endosomes
Article first published online: 13 JUN 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 42, Issue 8, pages 2187–2190, August 2012
How to Cite
Zehner, M., Rauen, J., Chasan, A. I., Embgenbroich, M., Camps, M. G., Kaden, S., Haas, A., Kurts, C., Endl, E., Beyer, M., Gröne, H.-J., Ossendorp, F. and Burgdorf, S. (2012), Intraendosomal flow cytometry: A novel approach to analyze the protein composition of antigen-loaded endosomes. Eur. J. Immunol., 42: 2187–2190. doi: 10.1002/eji.201142089
- Issue published online: 3 AUG 2012
- Article first published online: 13 JUN 2012
- Accepted manuscript online: 15 MAY 2012 01:29AM EST
- Manuscript Accepted: 27 APR 2012
- Manuscript Revised: 22 MAR 2012
- Manuscript Received: 6 SEP 2011
- German Research Foundation. Grant Numbers: SFB645, BU2441/1–1
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
Figure S1. Electron microscopy of enriched endosome preparation.Figure S2. Gating scheme of endosome preparations for flow cytometrical analysis.
Figure S3. Influence of saponin and Triton X-100 on isolated OVA containing endosomes.Figure S4: Influence of centrifugation on endosome clustering.Figure S5. Influence of centrifugation on endosome clustering.
Figure S6. Intracellular localization of MR-internalized OVA.
Figure S7. Time course experiments of endosomal maturation MR-deficient BM-M were incubated with fluorochrome-labeled OVA for 10 min and chased with medium for the indicated time points.Figure S8. Expression of Rab5 and LAMP1 on endosomes containing MR endocytosed OVA.
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