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Figure S1. Live CD4+ T-cell populations in M. avium infected mice. WT and nos2−/− mice were either left uninfected (UnInf) or infected (Inf) intravenously with 106 M. avium 25291 and the spleens, lungs and livers harvested. The organs were processed for flow cytometry and the (A, C) frequency and (B, D) number of live lymphocytes (LO) (A, B) and CD4+ T cells (C, D) within the organs determined. Cells were gated on live lymphocytes, doublet discrimination, and CD3+, CD4+ (n = 4–22, *p < 0.05, **p < 0.01, ***p < 0.001, by ANOVA).

Figure S2. Gating scheme for flow cytometric analysis and cell sorting. (A) The gating scheme for the detection of live, single cell, CD3+CD4+CD44+ T cells is shown in sequence. (B) Representative purity of the live, single cell (i) CD4+CD44+CD69hi and (ii) CD4+CD44+CD69lo cells sorted prior to RNA extraction.

eji2431-sup-0001-TableS1.xlsx5914KTable S1. RNA was extracted from purified populations of cells and analyzed using Affymetrix Mouse Genome 430 2.0 arrays as described in Materials and methods. Log intensity values for each spot and each population are shown below

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