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Supporting Information Fig. 1. Gating strategy for Treg-cell frequency determination.

Supporting Information Fig. 2. Frequencies of Treg cells in secondary lymphoid organs of B-cell-deficient μMT mice.

Supporting Information Fig. 3. Frequencies of Treg cells in secondary lymphoid organs of B-cell-deficient and B-cell-sufficient littermate mice.

Supporting Information Fig. 4. Frequencies of Treg cells in secondary lymphoid organs of 2-week-old C57BL/6 and B-cell-deficient JHT mice.

Supporting Information Fig. 5. Proliferation data of in vitro suppression assay.

Supporting Information Fig. 6. In vitro suppression assay with responder T cells and Treg cells from naive C57BL/6 and JHT mice.

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