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Table SI. Primers used for qPCR.

Figure S1. (A) The Ms4a8a promoter region defined on mouse chromosome 19, position 11157593 -11155594 [ENSEMBL database] was analyzed for transcription factor binding site with MATINSPECTOR 8.0.5 software package (Genomatix). By using a general core (threshold 0.75) and vertebrate promoter element (threshold 0.75) matrix a total of 596 consensus sites have been identified and displayed. Following transcription factor binding sites are labeled by colored bars: Six glucocorticoid response elements [GRE], seven ETS1 consensus sites (among them a single Spl1 site at position 1.4 kb). In addition, single motifs for STAT6, STAT3, AP1, and AP4 motif were detected. No binding sites of NFκB were detected. (B) Significant Ms4a8a expression in BMDM stimulated with LPS dexa/IL4 is achieved only after a 72h LPS stimulation period. BMDM were stimulated with control medium, dexa/IL4 and dexa/IL4 in addition to LPS for different time points (last 6h, last 24h, last 48h or last 72h). n = 3; Error bars show SEM. ** p < 0.01 according to Student's t-test. At least three independent experiments were performed.

Figure S2. MS4a8a and LPS induce a specific M2-associated gene profile in RAW264.7 macrophages. (A) A graphical heat map showing differentially expressed genes in a comparison of RAW264.7 cells transfected with either Ms4a8a [Ms4a8a], or control-DNA [empty vector, EV], and Ms4a8a-transfected [Ms4a8a_LPS], and control DNA transfected RAW264.7 cells [EV_LPS] upon treatment with 5 μg/ml LPS for 6 h. Differentially expressed genes have been selected by Significant rows with at least one -log10(p) -3.54. The individual expression (n = 3) of each stimulation is shown. Red color indicate up-regulation, blue color indicate down-regulation in relation to mean values. (B–C) Expression analysis of of Hdc (B) and Tcfec (C) of Ms4a8a-transfected and control DNA transfected RAW264.7 cells upon 6 h treatment with LPS assessed by qRT-PCR (n = 5). The p-value was (** p < 0.01) according to Student's t-test.

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