These authors contributed equally to this work.
The CD20 homolog Ms4a8a integrates pro- and anti-inflammatory signals in novel M2-like macrophages and is expressed in parasite infection
Version of Record online: 5 SEP 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 42, Issue 11, pages 2971–2982, November 2012
How to Cite
Schmieder, A., Schledzewski, K., Michel, J., Schönhaar, K., Morias, Y., Bosschaerts, T., Van den Bossche, J., Dorny, P., Sauer, A., Sticht, C., Géraud, C., Waibler, Z., Beschin, A. and Goerdt, S. (2012), The CD20 homolog Ms4a8a integrates pro- and anti-inflammatory signals in novel M2-like macrophages and is expressed in parasite infection. Eur. J. Immunol., 42: 2971–2982. doi: 10.1002/eji.201142331
- Issue online: 29 OCT 2012
- Version of Record online: 5 SEP 2012
- Accepted manuscript online: 18 JUL 2012 01:48AM EST
- Manuscript Accepted: 10 JUL 2012
- Manuscript Revised: 14 JUN 2012
- Manuscript Received: 14 DEC 2011
- Deutsche Forschungsgemeinschaft. Grant Numbers: SFB405, SFB-TR23, SFB938
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
Table SI. Primers used for qPCR.
Figure S1. (A) The Ms4a8a promoter region defined on mouse chromosome 19, position 11157593 -11155594 [ENSEMBL database] was analyzed for transcription factor binding site with MATINSPECTOR 8.0.5 software package (Genomatix). By using a general core (threshold 0.75) and vertebrate promoter element (threshold 0.75) matrix a total of 596 consensus sites have been identified and displayed. Following transcription factor binding sites are labeled by colored bars: Six glucocorticoid response elements [GRE], seven ETS1 consensus sites (among them a single Spl1 site at position 1.4 kb). In addition, single motifs for STAT6, STAT3, AP1, and AP4 motif were detected. No binding sites of NFκB were detected. (B) Significant Ms4a8a expression in BMDM stimulated with LPS dexa/IL4 is achieved only after a 72h LPS stimulation period. BMDM were stimulated with control medium, dexa/IL4 and dexa/IL4 in addition to LPS for different time points (last 6h, last 24h, last 48h or last 72h). n = 3; Error bars show SEM. ** p < 0.01 according to Student's t-test. At least three independent experiments were performed.
Figure S2. MS4a8a and LPS induce a specific M2-associated gene profile in RAW264.7 macrophages. (A) A graphical heat map showing differentially expressed genes in a comparison of RAW264.7 cells transfected with either Ms4a8a [Ms4a8a], or control-DNA [empty vector, EV], and Ms4a8a-transfected [Ms4a8a_LPS], and control DNA transfected RAW264.7 cells [EV_LPS] upon treatment with 5 μg/ml LPS for 6 h. Differentially expressed genes have been selected by Significant rows with at least one -log10(p) -3.54. The individual expression (n = 3) of each stimulation is shown. Red color indicate up-regulation, blue color indicate down-regulation in relation to mean values. (B–C) Expression analysis of of Hdc (B) and Tcfec (C) of Ms4a8a-transfected and control DNA transfected RAW264.7 cells upon 6 h treatment with LPS assessed by qRT-PCR (n = 5). The p-value was (** p < 0.01) according to Student's t-test.
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