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Figure S1. Effect of various TLRs ligands on reporter gene activity in HT-29 (A) and Caco-2 (B) cells. Reporter gene activity was measured after 24 h stimulation. Different letters indicate statistically different results with a p value <0.05; Student's T test.

Figure S2. Response of TSLP reporter clone (Caco-2) to butyrate (2 mM) and different concentration of Trichostatin (TSA) tested alone or in combination with IL-1 (10 ng/ml) or PMA (1ìM). Reporter gene activity was measured after 24 h stimulation. Results are mean ± SD of triplicate measurements of a representative of three independent experiments. ** = p < 0.01, *** = p < 0.001.

Figure S3. TSLP promoter-driven luciferase activity measured using a 3 kb-long promoter construct. Different letters indicate statistically different results with a p value <0.05; Student's T test.

Figure S4. Effect of Flagellin on NF1 or/and NF2 mutations of the TSLP promoter-driven luciferase activity. Different letters indicate statistically different results with a p value <0.05; Student's T test.

Figure S5. Characterization of NF1 and NF2 binding site by promoter deletion and site directed mutagenesis on various epithelial cell lines. HeLa (a), HEK 293 (b), A549 (c). Results are mean ± standard deviation (SD) of triplicate measurements of a representative of three independent experiments. Different letters indicate statistically different results with a p value <0.05; Student's T test.

Figure S6. Effect of PMA (a), Butyrate (b) and combination of both (c) on TSLP promoter-driven luciferase activity. Various 5'deletions of the TSLP promoter were cloned in pCDNA3.1-Luc and transiently transfected in Caco-2 cells. Length of each construct is indicated. Cells were stimulated 24 h after transfection and reporter gene activity was assayed 24 h after stimulation. Results are mean ± standard deviation (SD) of triplicate measurements of a representative of three independent experiments. Different letters indicate statistically different results with a p value <0.05; Student's T test.

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