Identification of novel glycolipid ligands activating a sulfatide-reactive, CD1d-restricted, type II natural killer T lymphocyte
Article first published online: 22 AUG 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 42, Issue 11, pages 2851–2860, November 2012
How to Cite
Rhost, S., Löfbom, L., Rynmark, B.-M., Pei, B., Månsson, J.-E., Teneberg, S., Blomqvist, M. and Cardell, S. L. (2012), Identification of novel glycolipid ligands activating a sulfatide-reactive, CD1d-restricted, type II natural killer T lymphocyte. Eur. J. Immunol., 42: 2851–2860. doi: 10.1002/eji.201142350
- Issue published online: 29 OCT 2012
- Article first published online: 22 AUG 2012
- Accepted manuscript online: 10 JUL 2012 04:35AM EST
- Manuscript Accepted: 4 JUL 2012
- Manuscript Revised: 1 JUN 2012
- Manuscript Received: 22 DEC 2011
- Strategic Research Center for Mucosal Immunobiology and Vaccines (MIVAC)
- Swedish Cancer Foundation
- Swedish Research Council
- LUA-ALF Göteborg to S.C.
- NIH. Grant Number: AI45053
- Sahlgrenska Academy, University of Gothenburg
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
|eji2397-sup-0001-figures1.pdf||342K||Supplemental Figure 1. Flow cytometry profiles of co-stimulatory and co-inhibitory molecules expressed by JawsII and A20CD1d cells. (A) Black histograms indicate specific staining and white histograms show control antibody staining. (B) Filled histograms indicate specific staining and empty histograms show control antibody staining. Data are representative of two experiments. Supplemental Figure 2. Type II NKT hybridoma 14S.15.5D was activated by lyso-GalCer. CD1d-transfected APC were pulsed with lyso-GalCer or vehicle (DMSO) as described before, washed and co-cultured with 14S.15.5D hybridoma cells over night (Blomqvist et al. 2009, Eur J Immunol 39:1726). IL-2 in culture supernatants was measured by ELISA. Data indicate mean and SD of triplicate cultures and are representative of three experiments. Supplemental Figure 3. CD1d expression of APC. Cell lines were stained with anti-CD1d or control antibody. Upper plot: filled histograms (overlapping to the right) indicate CD1d expression, and empty histograms (overlapping to the left) show control antibody staining. Lower plots: filled histograms (overlapping to the right) indicate CD1d expression, and empty histograms (overlapping to the left) show control antibody staining.|
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