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eji2397-sup-0001-figures1.pdf342KSupplemental Figure 1. Flow cytometry profiles of co-stimulatory and co-inhibitory molecules expressed by JawsII and A20CD1d cells. (A) Black histograms indicate specific staining and white histograms show control antibody staining. (B) Filled histograms indicate specific staining and empty histograms show control antibody staining. Data are representative of two experiments. Supplemental Figure 2. Type II NKT hybridoma 14S.15.5D was activated by lyso-GalCer. CD1d-transfected APC were pulsed with lyso-GalCer or vehicle (DMSO) as described before, washed and co-cultured with 14S.15.5D hybridoma cells over night (Blomqvist et al. 2009, Eur J Immunol 39:1726). IL-2 in culture supernatants was measured by ELISA. Data indicate mean and SD of triplicate cultures and are representative of three experiments. Supplemental Figure 3. CD1d expression of APC. Cell lines were stained with anti-CD1d or control antibody. Upper plot: filled histograms (overlapping to the right) indicate CD1d expression, and empty histograms (overlapping to the left) show control antibody staining. Lower plots: filled histograms (overlapping to the right) indicate CD1d expression, and empty histograms (overlapping to the left) show control antibody staining.

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