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Figure 1A, 1B and 1 C. For the expression analysis of AdipoR1 and R2 „doublets “and dead cells were excluded. Human PBMCs were gated on CD3-CD56+ NK cells and for CD3+CD4+ T cells (Fig. 1A). Further CD56+ cells were subdivided into CD56high and CD56dim cells. Surface and intracellular expression of AdipoR1/R2 was analyzed.

Figure 2A, B, C, D and E. For identification of IFN-γ producing and CD107a+ degranulating cells, CD3-CD56+ cells were used. Dead cells and doublets were excluded from alalysis. INF-γ and CD107a was analyzed on CD56+ NK cells.For identification of sorted IFN-γ producing NK cells, dead cells, doublets and remained CD3+ cells were excluded. IFN-γ secretion was analyzed (Fig. 2B, D).

Figure 2F. For the analysis of cytotoxicity, K562 were labeled with a fluorescence dye and incubated with sorted NK cells. PI was used to gate on dead cells.

Figure 3A. For the identification of of DX5+Nkp46+ cells, ex vivo splenocytes of uninfected and infected WT and APN-KO mice were used. Doublets and CD3+ cells were excluded.

Figure 3B and C. For the identification of NK cell frequencies and numbers, ex vivo splenocytes of uninfected and infected WT and APN-KO mice were used. Doublets and CD3+ cells were excluded.

Figure 4. Intra- and extracellular AdipoR1 and 2 expression was analyzed on CD3e+ and CD3e-Nkp46+ splenocytes. Doublets were excluded from analysis.

Figure 5. Expression of the degranulation marker CD107a, NKG2D and CD94low/high was analyzed on CD3- Nkp46+ splenoycytes. In the flow cytometric analysis for CD94 expression we included the maturation marker CD11b. NK cell subsets were analyzed by CD11b and CD27 on Nkp46+ cells.

Figure 6. Expression of IFN-γ was analyzed ex vivo on CD3e-Nkp46+ splenocytes.

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