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Figure S1. NK cell KIR and NKG2A expression in CMVseropositive and seronegative donors PBMCs from CMV-seropositive (CMV+) or CMV-seronegative (CMV-) donors were stained for cell surface expression of the inhibitory receptors (A) KIR2DL1, (B) KIR2DL2/3, (C) KIR2DL5, (D) KIR3DL1 and of the activating receptors (E) KIR2DS1, (H) KIR2DS4, and (J) KIR3DS1 after gating on CD56+/CD3- NK cells. mRNA quantity was compared for the activating receptors KIR2DS2, KIR2DS3, and KIR2DS5 in immunomagnetically sorted NK cells by qRT-PCR. Data represent 6 experiments performed in 54 donors. Expression of each KIR is shown only in donors that carry the respective KIR gene. Horizontal lines represent means. Comparison between groups was made by Student's T-test.

Figure S2. Expansion of NK cells after co-culture with CMVinfected fibroblasts Total number of NK cells derived from CMV-seropositive (CMV+) and CMV seronegative donors (CMV-) was assessed during three-week culture in the presence of the fibroblast cells infected or not (control) with CMV by a combination of trypan blue cell counting and FACS phenotyping. Data are pooled from 2 experiments involving a total of 10 donors. Bars represent means and whiskers the standard error of the mean. Comparison between groups was made by Student's T-test.

Figure S3. Expression of KIR and NKG2A in FACS-sorted NK cells co-cultured with CMV-infected fibroblasts FACS-sorted NK cells from CMV-seropositive donors were co-cultured for 21 days with fibroblasts in the presence or absence of CMV and the expression of inhibitory KIR- and NKG2A receptors was compared by flowcytometry in cultured samples. Data are pooled from 2 experiments involving a total of 5 donors. Bars represent means and whiskers the standard error of the mean.

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