Regular Article

CTL recognition of HIV-1-infected cells via cross-recognition of multiple overlapping peptides from a single 11-mer Pol sequence

  1. Masao Hashimoto1,†,
  2. Tomohiro Akahoshi1,†,
  3. Hayato Murakoshi1,
  4. Naoki Ishizuka2,
  5. Shinichi Oka1,3,
  6. Masafumi Takiguchi1,*

Article first published online: 6 AUG 2012

DOI: 10.1002/eji.201242483

Issue

European Journal of Immunology

European Journal of Immunology

Volume 42, Issue 10, pages 2621–2631, October 2012

Additional Information

How to Cite

Hashimoto, M., Akahoshi, T., Murakoshi, H., Ishizuka, N., Oka, S. and Takiguchi, M. (2012), CTL recognition of HIV-1-infected cells via cross-recognition of multiple overlapping peptides from a single 11-mer Pol sequence. Eur. J. Immunol., 42: 2621–2631. doi: 10.1002/eji.201242483

Author Information

  1. 1

    Center for AIDS Research, Kumamoto University, Kumamoto, Japan

  2. 2

    Sanofi-Aventis, Tokyo, Japan

  3. 3

    AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan

  1. †

    These authors contributed equally to the work.

* Full correspondence: Prof. Masafumi Takiguchi, Takiguchi Project Laboratory, Center for AIDS Research, Kumamoto University, 2–2-1 Honjo, Kumamoto 860–0811, Japan
Fax:+81-96-373-6532
e-mail: masafumi@kumamoto-u.ac.jp

  1. †

    These authors contributed equally to the work.

Publication History

  1. Issue published online: 8 OCT 2012
  2. Article first published online: 6 AUG 2012
  3. Accepted manuscript online: 27 JUN 2012 11:53PM EST
  4. Manuscript Accepted: 21 JUN 2012
  5. Manuscript Revised: 24 MAY 2012
  6. Manuscript Received: 21 FEB 2012

Funded by

  • Global COE program “Global Education and Research Center Aiming at the Control of AIDS
  • Ministry of Education, Science, Sports, and Culture, Japan. Grant Number: H21-AIDS-010, H24-AIDS-007
  • Ministry of Health, Japan. Grant Number: 20390134
  • Ministry of Education, Science, Sports and Culture, Japan

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (1197K)

Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.

FilenameFormatSizeDescription
eji2382-sup-0001-S1.pdf139KFigure S1. Effect of incubation time in the ELISPOT assay on CD8+ T cell responses. Ex vivo PBMC from KI-676 was analyzed by conducting the ELISPOT assay using overlapping 8-mer to 11-mer peptides at a 100 nM concentration. The PBMC was cultured with each peptide for 16 or 40 hours. The error bars represent standard deviations, n = 3. Figure S2. Ability of overlapping 8-mer to 11-mer peptide-specific CD8+ T cells to recognize FV9 or FP10 peptide. Ex vivo PBMCs from KI-627 were analyzed by staining with a 100 nM concentration of PE-conjugated FV9- or FP10-specific tetramers. Table S1. Statistical analysis of the recognition ability of the CTL clones to the overlapping peptides at concentration of 1 nM

Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

View Full Article with Supporting Information (HTML) Get PDF (1197K)