Predominance of heterosubtypic IFN-γ-only-secreting effector memory T cells in pandemic H1N1 naive adults
Article first published online: 15 AUG 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 42, Issue 11, pages 2913–2924, November 2012
How to Cite
Sridhar, S., Begom, S., Bermingham, A., Ziegler, T., Roberts, K. L., Barclay, W. S., Openshaw, P. and Lalvani, A. (2012), Predominance of heterosubtypic IFN-γ-only-secreting effector memory T cells in pandemic H1N1 naive adults. Eur. J. Immunol., 42: 2913–2924. doi: 10.1002/eji.201242504
- Issue published online: 29 OCT 2012
- Article first published online: 15 AUG 2012
- Accepted manuscript online: 10 JUL 2012 04:35AM EST
- Manuscript Accepted: 29 JUN 2012
- Manuscript Revised: 30 MAY 2012
- Manuscript Received: 1 MAR 2012
- National Institute of Health Research
- Medical Research Council
- Imperial College Healthcare NHS Trust
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
Fig. S1. Individual level heterogeneity of the proportion of the cytokine secreting subsets to core proteins of pH1N1. Proportion of the total number of cytokine-secreting cells in ex vivo PBMCs for (A, E) PB1, (B, F) M1, (C, G) NP and (D, H) the summed response to PB1, M1 and NP antigens from the IFN-ã-only, IL-2-only and IFN- ã/IL-2 dual secreting subsets in pH1N1 sero-negative individuals was determined by Fluorescence-Immunospot. Individuals represented by symbols (A-D) or bars (E-H) and horizontal lines represent median responses. Individuals are not shown in the same order in E-H (n = 33).
Fig. S2. Gating strategy for Figure 4. Cells were gated based on side scatter and forward scatter to exclude debris followed by gating to exclude doublets, dead cells and non-lymphocyte cell populations. Cells were then gated for CD3 and CD3+ cells were further identified as CD4+CD8- and CD4-CD8+. For each of these populations, cells were divided into different effector and memory populations using CCR7 and CD45RA surface markers. For each of CCR7+CD45RA-, CCR7+CD45RA+, CD45RA+CCR7-, cells were gated for secretion of IFN-ã and IL-2.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.