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Figure 1S. Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4 for six days and were incubated or not of neutralizing anti-IL10 Abs within the first 24 h of culture. Thereafter, CD1a and DC-SIGN expression was analyzed by flow cytometry. (A) Surface CD1a and DC-SIGN expression of CD16- Mos, CD16+ Mos, CD16+ Mos plus anti-IL10 Abs or isotype (fill). A representative histogram is shown. (B) Results are expressed as the percentage of CD1a+CD14- DCs and shown as mean + SEM from four donors tested in two independent experiments.

Figure 2S. (A) Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4. On day 6 the percentages of viable cells recovered were determined by counting Trypan Blue nonstained cells in the Neubauer chamber. Results are mean + SEM of 12 donors in six independent experiments. Statistical significance for CD16+ Mos vs CD16- Mos (*p < 0.05) was determined by Wilcoxon test (B) Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4 for 24 h and Mo apoptosis/necrosis was assessed by surface staining with FITC-conjugated Annexin-V combined with propidium iodide staining. Results are shown as mean – SEM from six donors analyzed in three independent experiments.

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