Impaired dendritic cell differentiation of CD16-positive monocytes in tuberculosis: Role of p38 MAPK
Version of Record online: 14 JAN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 2, pages 335–347, February 2013
How to Cite
Balboa, L., Romero, M. M., Laborde, E., Sabio y García, C. A., Basile, J. I., Schierloh, P., Yokobori, N., Musella, R. M., Castagnino, J., de la Barrera, S., Sasiain, M. C. and Alemán, M. (2013), Impaired dendritic cell differentiation of CD16-positive monocytes in tuberculosis: Role of p38 MAPK. Eur. J. Immunol., 43: 335–347. doi: 10.1002/eji.201242557
- Issue online: 14 FEB 2013
- Version of Record online: 14 JAN 2013
- Accepted manuscript online: 27 NOV 2012 07:19PM EST
- Manuscript Accepted: 22 NOV 2012
- Manuscript Revised: 3 NOV 2012
- Manuscript Received: 22 MAR 2012
- Agencia Nacional de Promoción Científica y Tecnológica. Grant Numbers: PAE-PICT 2328, PAE-PICT 2329, PICT 2010-059
- Consejo Nacional de Investigaciones científicas y Técnicas. Grant Number: PIP 112-2008-01-01476
- Fundación Alberto J. Roemmers
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Figure 1S. Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4 for six days and were incubated or not of neutralizing anti-IL10 Abs within the first 24 h of culture. Thereafter, CD1a and DC-SIGN expression was analyzed by flow cytometry. (A) Surface CD1a and DC-SIGN expression of CD16- Mos, CD16+ Mos, CD16+ Mos plus anti-IL10 Abs or isotype (fill). A representative histogram is shown. (B) Results are expressed as the percentage of CD1a+CD14- DCs and shown as mean + SEM from four donors tested in two independent experiments.
Figure 2S. (A) Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4. On day 6 the percentages of viable cells recovered were determined by counting Trypan Blue nonstained cells in the Neubauer chamber. Results are mean + SEM of 12 donors in six independent experiments. Statistical significance for CD16+ Mos vs CD16- Mos (*p < 0.05) was determined by Wilcoxon test (B) Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4 for 24 h and Mo apoptosis/necrosis was assessed by surface staining with FITC-conjugated Annexin-V combined with propidium iodide staining. Results are shown as mean – SEM from six donors analyzed in three independent experiments.
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