Impaired dendritic cell differentiation of CD16-positive monocytes in tuberculosis: Role of p38 MAPK
Article first published online: 14 JAN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 2, pages 335–347, February 2013
How to Cite
Balboa, L., Romero, M. M., Laborde, E., Sabio y García, C. A., Basile, J. I., Schierloh, P., Yokobori, N., Musella, R. M., Castagnino, J., de la Barrera, S., Sasiain, M. C. and Alemán, M. (2013), Impaired dendritic cell differentiation of CD16-positive monocytes in tuberculosis: Role of p38 MAPK. Eur. J. Immunol., 43: 335–347. doi: 10.1002/eji.201242557
- Issue published online: 14 FEB 2013
- Article first published online: 14 JAN 2013
- Accepted manuscript online: 27 NOV 2012 07:19PM EST
- Manuscript Accepted: 22 NOV 2012
- Manuscript Revised: 3 NOV 2012
- Manuscript Received: 22 MAR 2012
- Agencia Nacional de Promoción Científica y Tecnológica. Grant Numbers: PAE-PICT 2328, PAE-PICT 2329, PICT 2010-059
- Consejo Nacional de Investigaciones científicas y Técnicas. Grant Number: PIP 112-2008-01-01476
- Fundación Alberto J. Roemmers
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
Figure 1S. Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4 for six days and were incubated or not of neutralizing anti-IL10 Abs within the first 24 h of culture. Thereafter, CD1a and DC-SIGN expression was analyzed by flow cytometry. (A) Surface CD1a and DC-SIGN expression of CD16- Mos, CD16+ Mos, CD16+ Mos plus anti-IL10 Abs or isotype (fill). A representative histogram is shown. (B) Results are expressed as the percentage of CD1a+CD14- DCs and shown as mean + SEM from four donors tested in two independent experiments.
Figure 2S. (A) Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4. On day 6 the percentages of viable cells recovered were determined by counting Trypan Blue nonstained cells in the Neubauer chamber. Results are mean + SEM of 12 donors in six independent experiments. Statistical significance for CD16+ Mos vs CD16- Mos (*p < 0.05) was determined by Wilcoxon test (B) Magnetically isolated CD16- or CD16+ Mos from HSs were cultured with GM-CSF and IL-4 for 24 h and Mo apoptosis/necrosis was assessed by surface staining with FITC-conjugated Annexin-V combined with propidium iodide staining. Results are shown as mean – SEM from six donors analyzed in three independent experiments.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.