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Figure 1. Subjects with detectable frequencies of H5N1-lgG MBCs at baseline develop large pool of H5N1-lgG MBCs but not of H1N1-lgG MBCs after vaccination Frequency of (A) H5N1- and (B) H1N1- specific IgG MBCs detected in subjects with (a) or without (b) measurable baseline H5N1 -IgG MBCs from the AT and TA (a n=27; b n=33) vaccination groups at baseline (day 1), and at three weeks after the first (day 21) and the second (day 43) vaccine dose. Each symbol represents an individual subject, the line across the box plots identifies the median, and the box and whiskers plots represent the interquartile range and maximum and minimum respectively. The grey line across each plot identifies the 'grand mean' of all values. Differences between a and b groups were analyzed by the Wilcoxon/Kruskal-Wallis Tests. P-values from the 1 -way ChiSquare approximation tests are indicated inside each plot. Each symbol represents an individual subject from 60 analyzed.

Figure 2. The expansion of H5N1-MBCs does not strictly associate with the size of the H1N1-MBC pool at baseline while it relates inversely with the baseline size of the H5N1-MBC pool. A: Distribution of H5N1-MBC fold increase after one vaccine dose across the H1N1-High (closed symbols; N=30) or the H1N1-Low group (open symbols; N=30). Black lines represent median values for each group (p=0.57 by Wilcoxon/Kruskal-Wallis test). B: Scatter plot of paired logl O-transformed values of fold increase in H5N1 -IgG MBC frequencies (day 21 over day 1, y-axis) and baseline frequencies of H5N1 -IgG MBCs (x-axis). C: Scatter plot of paired values of fold increase in H1N1-lgG MBC frequencies (day 21 over day 1, y-axis) and baseline frequencies of H1N1-lgG MBCs (x-axis). H1N1-High subjects are depicted in each plot with closed symbols (N=30) and H1N1 -Low subjects with open symbols (N=30). Shown are the regression lines with related 95% confidence intervals (gray areas), slope, intercepts, R2 and p-values. Each dot represents one single subject from 60 analyzed

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