Human antibodies reactive to NeuGcGM3 ganglioside have cytotoxic antitumor properties
Article first published online: 8 FEB 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 3, pages 826–837, March 2013
How to Cite
Rodríguez-Zhurbenko, N., Martínez, D., Blanco, R., Rondón, T., Griñán, T. and Hernández, A. M. (2013), Human antibodies reactive to NeuGcGM3 ganglioside have cytotoxic antitumor properties. Eur. J. Immunol., 43: 826–837. doi: 10.1002/eji.201242693
- Issue published online: 11 MAR 2013
- Article first published online: 8 FEB 2013
- Accepted manuscript online: 14 JAN 2013 09:04AM EST
- Manuscript Accepted: 8 JAN 2013
- Manuscript Revised: 19 NOV 2012
- Manuscript Received: 22 MAY 2012
- Center of Molecular Immunology
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Figure S1. Level of anti-NeuGcGM3 antibodies in male and female healthy humans is similar.
Figure S2. Total amount of IgM and IgG in healthy donors’ sera does not change with age.
Figure S3. Presence of NeuGcGM3 on L1210 cells.
Figure S4. Healthy humans’ sera induced complement mediated cell death to NeuGcGM3 expressing tumor cells.
Figure S5. Induced complement independent cell death positively correlates with both the levels of anti-NeuGcGM3 antibodies and tumor cell binding.
Figure S6. Incubation of L1210 cells with cytotoxic healthy humans’ sera did not induce caspase 3 activation.
Figure S7. Anti-NeuGcGM3 Abs obtained from NSCLC patients demonstrate specific binding.
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