The IL-36 receptor pathway regulates Aspergillus fumigatus-induced Th1 and Th17 responses
Version of Record online: 18 DEC 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 2, pages 416–426, February 2013
How to Cite
Gresnigt, M. S., Rösler, B., Jacobs, C. W.M., Becker, Katharina. L., Joosten, L. A.B., van der Meer, J. W.M., Netea, M. G., Dinarello, C. A. and van de Veerdonk, F. L. (2013), The IL-36 receptor pathway regulates Aspergillus fumigatus-induced Th1 and Th17 responses. Eur. J. Immunol., 43: 416–426. doi: 10.1002/eji.201242711
- Issue online: 14 FEB 2013
- Version of Record online: 18 DEC 2012
- Accepted manuscript online: 12 NOV 2012 06:24AM EST
- Manuscript Accepted: 5 NOV 2012
- Manuscript Revised: 28 SEP 2012
- Manuscript Received: 30 MAY 2012
- Netherlands Organization for Scientific Research
- NIH. Grant Numbers: AI-15614, AR-45584
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Figure S1. IL-17, IL-22, and IFN-γ concentrations in culture supernatants of CD4 cells (5 × 105) stimulated for 7 days with medium, IL-36β or IL-36γ. Concentrations of IL-36 cytokines range from 1 to 100 ng/mL.
Figure S2. IL-12Rβ1, IL-1R1, and IL-18Rα expression on CD4 T cells and total PBMCs (n = 4 donors) stimulated for 7 days with A. fumigatus in the presence or absence of IL-36Ra (concentration ranging from 0.1 to 100 ng/mL). Whole PBMCs were stained with anti-CD4, anti-IL-12Rβ1, anti-IL-1R1, and anti-IL-18Rα. CD4 positive population was gated and subsequently the percentage of positive cells for each of the receptors was determined.
Figure S3. IL-18 and IL-12p35 mRNA expression in PBMCs stimulated for 24h with 1 × 107/mL Aspergillus conidia or hyphae in the presence or absence or IL-36Ra in concentrations ranging from 1 to 100 ng/mL.
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