Activated monocytes augment TRAIL-mediated cytotoxicity by human NK cells through release of IFN-γ
Article first published online: 26 OCT 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 1, pages 249–257, January 2013
How to Cite
Sarhan, D., D'Arcy, P., Wennerberg, E., Lidén, M., Hu, J., Winqvist, O., Rolny, C. and Lundqvist, A. (2013), Activated monocytes augment TRAIL-mediated cytotoxicity by human NK cells through release of IFN-γ. Eur. J. Immunol., 43: 249–257. doi: 10.1002/eji.201242735
- Issue published online: 16 JAN 2013
- Article first published online: 26 OCT 2012
- Accepted manuscript online: 19 SEP 2012 07:07AM EST
- Manuscript Accepted: 11 SEP 2012
- Manuscript Revised: 29 AUG 2012
- Manuscript Received: 10 JUN 2012
- Swedish Research Council
- Swedish Cancer Society
- European Research Council Society
- Karolinska Institutet
- Jeanssons Stiftelser
- Åke Wibergs Stiftelse
- Magnus Bergvalls Stiftelse
- Fredrik och Ingrid Thurings Stiftelse
- Stiftelsen Clas Groschinskys Minnesfond
- Swedish Society of Medicine
As a service to our authors and readers, this journal provides supporting information supplied by the authors.
Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset.
Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
Supporting Information Figure 1. NK cells were cultured for 7 days without feeder cells (A) or co-cultured with T cells, B cells or monocytes (B) in presence or absence of ZA and stained for CD56 and TRAIL (n=3). One of 3 independent experiments is shown for A. The fold-change in TRAIL expression was calculated between ZA-treated and untreated cultures. Boxes represent fold change values ranging from min to max and lines represent mean values. p-values were calculated by paired student's t-test from three pooled experiments. (C) NK cells were co-cultured with monocytes for 7 days in presence or absence of ZA and neutralizing antibodies against IFN-γ (αIFNγ) and stained for NKG2D (n=2). Data are shown as mean + SEM of results pooled from two experiments.
Supporting Information Figure 2. (A) A representative flow cytometry plots for gating strategy is shown. Monocytes, NK or T cells were isolated by magnetic beads and stained for CD56, CD3, CD14 to assess their purity on day 0 B) or day 7 after co-culture (C).
Supporting Information Figure 3. (A) Purified monocytes were cultured for 4 days in presence or absence of ZA and stained for CD54. (B) In vitro 18 hour 51Cr-release assay by recombinant TRAIL [100ng/ml] (r-TRAIL) against untreated (Untr) or b-AP15-treated J82 tumor cells. Data are shown as mean + SEM of results pooled from three experiments.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.