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Supporting Information Figure 1. NK cells were cultured for 7 days without feeder cells (A) or co-cultured with T cells, B cells or monocytes (B) in presence or absence of ZA and stained for CD56 and TRAIL (n=3). One of 3 independent experiments is shown for A. The fold-change in TRAIL expression was calculated between ZA-treated and untreated cultures. Boxes represent fold change values ranging from min to max and lines represent mean values. p-values were calculated by paired student's t-test from three pooled experiments. (C) NK cells were co-cultured with monocytes for 7 days in presence or absence of ZA and neutralizing antibodies against IFN-γ (αIFNγ) and stained for NKG2D (n=2). Data are shown as mean + SEM of results pooled from two experiments.

Supporting Information Figure 2. (A) A representative flow cytometry plots for gating strategy is shown. Monocytes, NK or T cells were isolated by magnetic beads and stained for CD56, CD3, CD14 to assess their purity on day 0 B) or day 7 after co-culture (C).

Supporting Information Figure 3. (A) Purified monocytes were cultured for 4 days in presence or absence of ZA and stained for CD54. (B) In vitro 18 hour 51Cr-release assay by recombinant TRAIL [100ng/ml] (r-TRAIL) against untreated (Untr) or b-AP15-treated J82 tumor cells. Data are shown as mean + SEM of results pooled from three experiments.

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