DX5+CD4+ T cells modulate CD4+ T-cell response via inhibition of IL-12 production by DCs
Version of Record online: 14 FEB 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 2, pages 439–446, February 2013
How to Cite
el Bannoudi, H., Han, W. G. H., Stoop, J. N., Louis-Plence, P., Huizinga, T. W. J. and Toes, R. E. M. (2013), DX5+CD4+ T cells modulate CD4+ T-cell response via inhibition of IL-12 production by DCs. Eur. J. Immunol., 43: 439–446. doi: 10.1002/eji.201242796
- Issue online: 14 FEB 2013
- Version of Record online: 14 FEB 2013
- Accepted manuscript online: 21 NOV 2012 03:08AM EST
- Manuscript Revised: 28 SEP 2012
- Manuscript Accepted: 15 SEP 2012
- Manuscript Received: 5 JUL 2012
- European Community's Seventh Framework Programme FP7. Grant Number: HEALTH-F2–2008-223404
- Centre for Medical Systems Biology (CMSB)
- Dutch Arthritis Foundation
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Supporting Information Figure S1: IL-10 production by DX5+CD4+ Tcells DX5+CD4+TcellsandDX5-CD4+ T cells isolated from BALB/c mice that have received 3 injections withim mature DCs, were stimulated with anti-CD3 and anti-CD28. After 3 days IL-10 production was a ssessed by flow cytometry. Each bar represents one mouse. The experiment is performed more than 3 times. DX5+CD4+ T cells and DX5-CD4+ T cells are from the same mouse
Supporting Information Figure S2: gating strategy for figure 2The expression of the surface molecules (PDL-1, PDL-2, CD40, CD80, CD86 andMHC class II) on the modified bone marrow derived DCs was analyzed after dead cells were excluded based on FSC and SSC. The same gating strategy is applied for other surface molecules
Supporting Information Figure S3: gating strategy for Figure 4 and 5CD4+T cells were isolated from D011.10 (OVA specific) mice. To identify IFN-γ production by OVA specific CD4+T cells, lymphocytes were first gated based on FSC and SSC, subsequently CD4 and KJ1-26 positive cells were gated and further analyzed for IFN-γ production.
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