Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation
Article first published online: 6 FEB 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 3, pages 595–605, March 2013
How to Cite
Moffat, J. M., Segura, E., Khoury, G., Caminschi, I., Cameron, P. U., Lewin, S. R., Villadangos, J. A. and Mintern, J. D. (2013), Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation. Eur. J. Immunol., 43: 595–605. doi: 10.1002/eji.201242799
- Issue published online: 11 MAR 2013
- Article first published online: 6 FEB 2013
- Accepted manuscript online: 9 JAN 2013 09:55AM EST
- Manuscript Accepted: 4 JAN 2013
- Manuscript Revised: 19 NOV 2012
- Manuscript Received: 5 JUL 2012
- International AIDS Society and National Institute of Health, USA
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Figure S1. C57BL/6 mice were immunized with 2 ìg of rat anti-BST-2 antibody (120-G8) or rat isotype control antibody (GL113) intravenously. Group A received antibody alone, Group B received antibody plus 20nmol CpG and Group C received 20nmol CpG 24 hours prior to antibody. Serum was collected 14 days following immunization and anti-rat IgG levels measured by ELISA. Positive control is a rat IgG positive serum from a previously immunised mouse. Each symbol represents an individual mouse and the bar designates the mean. This data is representative of two independent experiments.
Figure S2. Flow cytometry gating for isolation of mouse immune cells subsets. pDC: CD11c intermediate, CD45RA high, Ly6C high cells; CD8+ DC: CD11c high, CD8 high cells; CD8- DC: CD11c high, CD8 low; macrophages: CD11b high, F4/80 high; B cells: B220 high, CD19 high; CD4 T cells: CD3 high, CD4 high; CD8 T cells: CD3 high, CD8 high.
Figure S3. Flow cytometry gating for antigen presentation assays. OT-I: CD8+, TCRVα2+; OT-II: CD4+, TCR Vα 2+. Proliferating cells were determined as cells that had undergone division based on CFSE profiles.
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