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eji2497-sup-0001-FigureS1.pdf191KFigure S1. Flow cytometric gating strategy of CCR5 and CXCR4 receptor expression on CD4+/CD8+ BALMCs. (A) The gating strategy used to identify CD4+ and CD8+ T cells from BAL is shown in representative density plots from a single person. From left to right, lymphocytes were selected using forward scatter/side scatter-area, subsequently followed by gating on CD3+ T cells and thereafter selecting CD4+ and CD8+ T cells. (B–E) Representative histograms show (B, D) CCR5 and (C, E) CXCR4 expression on (B, C) CD4+ and (D, E) CD8+ BALMCs. The grey histograms give one representative example of an HIV-1-uninfected person, the black histograms show one representative example of an HIV-1-infected person. The same strategy was used for phenotyping of PBMCs.

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