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Supporting Information Figure 1. IL-4-treatment does not change PKD protein expression. B cells were cultured in medium alone or with IL-4 (10 ng/ml) for 24 hours. Whole cell extracts were western blotted with anti-PKD antibody. Blots were stripped and reprobed with anti-actin antibody. One of three comparable experiments is shown.

Supporting Information Figure 2. Minimum doses of inhibitors are sufficient to block BCR-initiated IκBα degradation. B cells were unstimulated (0) or stimulated with anti-Ig (15 μg/ml) for 45 min or 90 min. B cells were exposed to DMSO and cycloheximide (CHX, 20 μM), Ly294002 (20 μM) and CHX (20 μM), Go6976 (0.5 μM) and CHX (20 μM), Go6983 (2μM) and CHX (20 μM), or GF109203X (5 μM) and CHX (20 μM) for 60 min before addition of anti-Ig. Whole cell extracts were western blotted with anti-IκBα antibody. Blots were stripped and reprobed with anti-actin antibody. One of three comparable experiments is shown.

Supporting Information Figure 3. PKCδ/ε does not associate with PKD. B cells were cultured in medium alone or with IL-4 (10 ng/ml) for 24 hours and were stimulated with anti-Ig for 0 (-) or 5 (+) min in the presence or absence of Ly294002. Whole cell extracts were immunoprecipitated (IP) with a specific anti-PKD antibody. Immunoprecipitates or whole cell lysate (WC) as a positive control were western blotted with anti-PKCδ and anti-PKCε antibodies. Blots were stripped and reprobed with anti-PKD antibody. One of three comparable experiments is shown.

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