B-cell receptor signal strength influences terminal differentiation
Article first published online: 24 JAN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 3, pages 619–628, March 2013
How to Cite
Lechouane, F., Bonaud, A., Delpy, L., Casola, S., Oruc, Z., Chemin, G., Cogné, M. and Sirac, C. (2013), B-cell receptor signal strength influences terminal differentiation. Eur. J. Immunol., 43: 619–628. doi: 10.1002/eji.201242912
- Issue published online: 11 MAR 2013
- Article first published online: 24 JAN 2013
- Accepted manuscript online: 26 DEC 2012 08:47AM EST
- Manuscript Accepted: 19 DEC 2012
- Manuscript Revised: 11 NOV 2012
- Manuscript Received: 11 AUG 2012
- Conseil Régional du Limousin
- French government fellowships
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Table S1. Antibodies used in this study
Figure S1. Low level of surface BCR in κCH B cells.
Figure S2. B220+CD138low splenocytes do not harbor plasma cell features.
Figure S3. Injections of serum in DH−LMP2A mice restore normal level of IgG.
Figure S4. In vitro proliferation of B cells after 48 and 72 h of LPS stimulation was evaluated by CFSE staining for WT (shaded histogram), DH−LMP2A (light line) and VH−LMP2A (dark line). Data are representative of 3 independent experiments.
Figure S5: Effect of replacement of VH promoter by DH promoter on PC differentiation (top) and B-1 subset (bottom) was evaluated in vivo in VH−LMP2A-CreERT2 mice. Animals were treated for 10 days with tamoxifen or vehicle and spleen were analyzed by flow cytometry. Data are representative of 2 independent experiments with 2 mice for each condition.
Figure S6. Magnetically-purified B cell populations are devoid of PC.
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