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Table S1. Antibodies used in this study

Figure S1. Low level of surface BCR in κCH B cells.

Figure S2. B220+CD138low splenocytes do not harbor plasma cell features.

Figure S3. Injections of serum in DHLMP2A mice restore normal level of IgG.

Figure S4. In vitro proliferation of B cells after 48 and 72 h of LPS stimulation was evaluated by CFSE staining for WT (shaded histogram), DHLMP2A (light line) and VHLMP2A (dark line). Data are representative of 3 independent experiments.

Figure S5: Effect of replacement of VH promoter by DH promoter on PC differentiation (top) and B-1 subset (bottom) was evaluated in vivo in VHLMP2A-CreERT2 mice. Animals were treated for 10 days with tamoxifen or vehicle and spleen were analyzed by flow cytometry. Data are representative of 2 independent experiments with 2 mice for each condition.

Figure S6. Magnetically-purified B cell populations are devoid of PC.

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