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Figure S1. Coadministration of a poxviral vector skews the antibody response toward the induction of cytophilic antibody isotypes. Mice were immunized with 0.2 μg TIV ±MVA-NP+M1 or 1 μg TIV ±MVA-NP+M1. IgG1 and IgG2a were measured in the serum at the peak of the antibody response (8 wk) in response to TIV. Panel A: Isotype levels at two separate doses of TIV given via the mixed administration route (Materials and Methods). Panel B: IgG2a/IgG1 ratios were calculated and median responses are shown. Mann Whitney test was used to determine statistical significance (p<*0.05).

Figure S2. Characterization of the antigen specific T cell responses to H5 HA. C57BL/6 mice were immunized with 10 μg H5 HA (A/VN/1203/04) ± MVANP+ M1. Intracellular cytokine staining was performed on spleens 2 wk postimmunization. Graphs represent the frequency of IFN-γ producing T cells in response to H5 HA (VN/1203/04) peptide pools. Panel A: CD4+ T cells. Panel B CD8+ T cells.

Figure S3. Characterization of the antigen specific T cell responses to NP. C57BL/6 mice were immunized with 10 μg H5 HA (A/VN/1203/04) ± MVANP+ M1. Intracellular cytokine staining was performed on spleens 2 wk postimmunization. Graphs represent the frequency of IFN-γ producing T cells in response to a single NP peptide pool. Panel A: CD4+ T cells. Panel B: CD8+ T cells.

Figure S4. Gating Strategy for T cell characterization: Lymphocytes were gated based on side scatter and forward scatter followed by gating to exclude doublets. Cells were then identified as CD4+ or CD8+ and for each of these populations were further analyzed to determine the frequency of IFN-γ+ cells.

Figure S5. Gating Strategy Germinal Centers: Lymphocytes were gated based on forward and side scatter followed by gating to exclude doublets. B cells were identified as B220+ and cells were further analyzed for expression of germinal center markers GL7+ and CD95+.

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