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Figure S1. Numbers and frequencies of B cells and T cells in IL-10 deficient mice. Six-week-old IL-10FL/FL Cre- mice (white bars), IL-10FL/FL CD4-Cre+ mice (black bars), and IL-10FL/FL CD19- Cre+ mice were naturally infected with L. sigmodontis. Spleen cells were stained for CD4, CD19, CD8, Foxp3, DX5, and CD3 at day 60 p.i., and cellular composition of spleen cells was analysed by flow cytometry. Representative sets of blots are shown to identify (A) CD19+ B cells, (B) CD4+CD8- T helper cells, CD8+CD4- CTL, and CD4+Foxp3+ regulatory T cells, and (C) DX5+CD3- NK cells and DX5+CD3+ NKT cells in the lymphocyte gate. Shown are the mean numbers and frequencies of cell subtypes of 4 independent. Results are expressed as mean + SEM of n ≥ 9 as total number of mice per group across experiments. *p < 0.05, **p<0.01, ***p< 0.001 between means of CD4- and CD19-specific IL-10 deficient mice compared with the IL-10FL/FL Crecontrol group, ANOVA with Bonferroni posttest.

Figure S2. Humoral response of L. sigmodontis-infected mice with T-cell- and B-cell-specific IL-10 deficiency. L. sigmodontis-specific Ig (IgG1, IgG2b, IgM, and IgE) was quantified in sera from L. sigmodontis-infected IL- 10FL/FL Cre- (○ with dotted line), IL-10FL/FL CD4-Cre+ (▪ with black line), and IL-10FL/FL CD19-Cre+ mice (♦ with grey line) at indicated time points of infection (d0 to d60 p.i.) by ELISA. Results are expressed as arbitrary units (a.u.) (OD450 of samples subtracted by OD450 of blank). Serum dilutions were 1:1000 for IgG1 and IgM, and 1:100 for IgG2b and IgE. Graphs show combined results of 2 independent experiments (n ≥ 9). Error bars show SEM.

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