See accompanying Commentary: http://dx.doi.org/10.1002/eji.201343XXX
Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells
Article first published online: 26 FEB 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 4, pages 1063–1073, April 2013
How to Cite
Iwasaki, Y., Fujio, K., Okamura, T., Yanai, A., Sumitomo, S., Shoda, H., Tamura, T., Yoshida, H., Charnay, P. and Yamamoto, K. (2013), Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells. Eur. J. Immunol., 43: 1063–1073. doi: 10.1002/eji.201242942
- Issue published online: 16 APR 2013
- Article first published online: 26 FEB 2013
- Accepted manuscript online: 25 JAN 2013 06:01AM EST
- Manuscript Accepted: 22 JAN 2013
- Manuscript Revised: 14 JAN 2013
- Manuscript Received: 24 AUG 2012
- Japan Society for the Promotion of Science, Ministry of Health, Labor and Welfare
- Ministry of Education, Culture, Sports, Science and Technology
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Figure 1. The full gating strategy used in our experiments.
Cells were gated based on side scatter and forward scatter to exclude debris. Cells were then gated for CD4 and CD4+ cells and were divided using Egr-2 and LAG-3 expressions. To assessing proliferation, we labeled cells with CFSE at the start of the culture and the relationship between Egr-2 expression and the CFSE dilution level was examined in CD4+ cells.
(A) TheChIP assay result shown in Figure 2B was re-calculated. The result was presented as % input. (B) A gel picture of quantitative real-time PCR products. PCR products from Input DNA and immunoprecipitated DNA with anti-Egr-2 IgG or anti-control IgG amplified with the designed primers detecting Blimp-1 promoter sequences (# GPM1042845(-)01A; SA Biosciences) were subjected to gel electrophoresis. The amplicon size was 112 bp.
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