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Figure S1. Representative back-gating of CD8+ and CD4+ LP T cells. Lamina Propria Lymphocytes were gated by FSC-A and SSC-A, then the single cells were gated on by SSC-W. Next, dead cells were excluded by live-dead aqua staining and LPLs were gated on TCRb+ cells and then sub-gated into CD4+ and CD8+ populations.

Figure S2. CD4+, CD8+ and MHCII+ cell characterization in MHCII-/-gWT mice. A-B) Gated on live TCRb+ lymphocytes, dot plots depict the number of CD4+ and CD8+ T cells in the lamina propria of WT and MHCII-/- mice (A) or WTgWT and MHCII-/-gWT chimeric mice (B). C) Expression of MHC class II by flow cytometry on live lymphocytes in the spleen and lamina propria of WTgWT and MHCII-/-gWT chimeric mice. Dot plots represent one representative of 3 to 5 independent experiments.

Figure S3. Number of IL-17+ and IFNg+ CD4 T cells after infection with C. rodentium. The bar graphs depict the number of cells per cecum of IL-17A+ (A) and IFNg+ (B) CD4+ T cells in WT[RIGHTWARDS ARROW]WT and MHCII-/-[RIGHTWARDS ARROW]WT mice (average of 3 to 5 experiments). Statistical analysis was performed using a two-tailed student's t-test where: * = p < 0.05, ns = not significant.

Figure S4. Intracellular IL-22 expression in CD4+TCRb+ LPLs. A-B) Gated on live CD4+TCRb+ lymphocytes, dot plots depict the percentage of IL-17+ and IL-22+ CD4+T cells in the lamina propria of WT[RIGHTWARDS ARROW] WT and MHCII-/-[RIGHTWARDS ARROW]WT mice infected with C. rodentium for 4 days (A) or infected with Salmonella enterica serovar Typhimurium for 1 day (B). One representative of two independent experiments.

Figure S5. Characterization of CIITA-/-[RIGHTWARDS ARROW]WT chimeric mice. A) Domain organization of the Nod-like receptor CIITA: CARD = Caspase recruitment domain, TA = transcriptional activator domain, NBD = nucleotide binding domain and LRR = leucine-rich domain. B) Expression of MHC class II by flow cytometry on live lymphocytes in the lamina propria of WT[RIGHTWARDS ARROW]WT and CIITA-/-[RIGHTWARDS ARROW]WT chimeric mice. C) Survival curve of WT[RIGHTWARDS ARROW]WT and CIITA-/-[RIGHTWARDS ARROW]WT mice infected by oral gavage with 1×109 CFU of C. rodentium (4 to 6 mice per group).

Figure S6. Intracellular IL-22 expression in CD8+TCRb+ LPLs. Gated on live CD8+TCRb+ lymphocytes, dot plots depict the percentage of IL-17+ and IL-22+ CD8+T cells in the lamina propria of WT[RIGHTWARDS ARROW] WT and MHCII-/-[RIGHTWARDS ARROW]WT mice. One representative of two independent experiments.

Figure S7. CD44 expression on IL-17+, FOXP3+ and IFN+ CD8+ T cells in the lamina propria of MHCII-/-gWT mice. Dot plots depict the expression CD44, IL-17 (left), FOXP3 (middle) and IFN(right)on gated CD8+TCR+ LPLs isolated from uninfected MHCII-/-gWT mice. One representative of 3 independent experiments.

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