Cytolytic effector pathways and IFN-γ help protect against Japanese encephalitis
Article first published online: 18 JUN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 7, pages 1789–1798, July 2013
How to Cite
Larena, M., Regner, M. and Lobigs, M. (2013), Cytolytic effector pathways and IFN-γ help protect against Japanese encephalitis. Eur. J. Immunol., 43: 1789–1798. doi: 10.1002/eji.201243152
- Issue published online: 4 JUL 2013
- Article first published online: 18 JUN 2013
- Accepted manuscript online: 9 APR 2013 02:56AM EST
- Manuscript Accepted: 3 APR 2013
- Manuscript Revised: 5 FEB 2013
- Manuscript Received: 14 NOV 2012
- Australian National University, Australia
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Figure S1. Immunohistochemistry. Brain sections of mock infected Fas-/-xGzmA/B -/- mice, and Fas-/-xGzmA/B -/- and wild-type mice infected s.c. for 10 days with 103 PFU of JEV. Detection of JEV antigen by immunohistochemistry was performed with the use of a mAb that recognises the flavivirus NS1 protein, as previously described . Representative sections of the cerebral cortex, hippocampus, thalamus, cerebellum and brainstem are shown. Brown staining is indicative of infected cells.
Figure S2. JEV-specific Ab responses in WT and KO mice. Groups of 8-week-old mice were infected s.c. with 103 PFU of JEV, and serum samples collected at the indicated time points. Anti-JEV IgM (A), IgG1 (B) and IgG2b (C) isotype Ab titers were determined by ELISA. The data presented are reciprocal mean endpoint titers representative of four mice per time point with the SEM indicated by error bars. (D) Neutralizing antibody titers determined by plaque reduction neutralization assay. The data presented are mean PRNT50 titers representative of five mice per time point with the SEM indicated by error bars. Differences between WT and KO mouse groups were not significant (p > 0.05; twotailed Mann–Whitney test).
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