These authors contributed equally to this work.
Dendritic cell homeostasis is maintained by nonhematopoietic and T-cell-produced Flt3-ligand in steady state and during immune responses
Version of Record online: 27 APR 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 6, pages 1651–1658, June 2013
How to Cite
Saito, Y., Boddupalli, C. S., Borsotti, C. and Manz, M. G. (2013), Dendritic cell homeostasis is maintained by nonhematopoietic and T-cell-produced Flt3-ligand in steady state and during immune responses. Eur. J. Immunol., 43: 1651–1658. doi: 10.1002/eji.201243163
- Issue online: 21 JUN 2013
- Version of Record online: 27 APR 2013
- Accepted manuscript online: 21 MAR 2013 04:40AM EST
- Manuscript Accepted: 18 MAR 2013
- Manuscript Revised: 1 FEB 2013
- Manuscript Received: 17 NOV 2012
- Uehara Memorial Foundation
- Swiss National Science Foundation. Grant Number: 310030-116637
- European Commission FP6 Network of Excellence initiative. Grant Number: LSHB-CT-2004-512074
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Figure S1. Flt3L mRNA expression in lymphoid tissues and hematopoietic cells. (A) Graph shows Flt3L mRNA expression in steady-state organs as indicated. (B) Bar graph shows Flt3L mRNA expression in sorted cell populations from spleen and LN. Experiment was repeated twice, sorted cells were pooled from 2-3 mice in each experiment. *P < .05; **P < .01; ***P < .001. NS (not significant).
Figure S2. M-CSF and GM-CSF mRNA expression in the draining lymph nodes. Graph shows M-CSF (Csf-1) or GM-CSF (Csf-2) mRNA expression relative to β-actin or 18s mRNA expression, respectively, in the draining LN after OVA or PBS stimulation. The data shown were obtained from 2 independent experiments (n = 3-5 per group).
Figure S3. Gating strategies used in the flow cytometry data in this work. (A) Gating strategy for donor-derived cDC and pDC in the recipient spleen. (B-D) Gating strategy for CFSE-labeled T cells (B), cDC , mDC (C) and pre-cDC (D) in the draining LNs. FSC, Forward scatter; SSC, Side scatter: Lin, Lineage.
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